| Objective Metabolomics study on Ifosfamide induced liver injury in mice to determine the biological markers of chemotherapy liver injury; based on these markers, combining the pathology to compare the different dose of Bupleuri sarmentosum decoction treatment on liver injury; clarifying the protective mechanism.Methods Using GC-MS technique to analyses the metabolite profiling of liver homogenates in mouse; using multivariate pattern recognition method to analyses the metabolite spectra difference in normal and liver injured mouse, finding the potential biomarkers; comparing the changes of these metabolites by Bupleurum sarmentosum decoction treatments; combined with pathological analysis, clarifying the protection mechanism of Bupleurum sarmentosum decoction treatment on Ifosfamide induced liver injury.Results①large and middle dose of Chinese medicine treatment group could effectively degrade the ifosfamide induced ALT, AST, ADH increases;②the endogenous biomarkers that could characterize the ifosfamide induced liver injury was selected, where different dose of Bupleuri sarmentosum decoction treatments could significantly change the metabolite profiling of endogenous biological markers, whose metabolic distribution tended to be normal;③The Ifosfamide induced liver injury was characterized by liver cell edema that was widely scattered on liver cell to apoptosis; different dose of Bupleuri sarmentosum decoction treatments had significantly reduced the edema and hepatocyte apoptosis in mouse liver cells.Conclusion①Bupleuri sarmentosum decoction could reduce the increase of chemotherapy drugs ifosfamide induced ALT, AST and ALP, correcting the disorders of liver metabolites;②Lactic acid, beta hydroxy butyric acid, urea,2-keto-L-gulonic acid, N-acetyl glucosamine, linoleic acid, stearic acid, uridine and cholesterol were characterized as metabolic markers of ifosfamide induced liver injury. |
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