The Reasearch Of A Rat Model Of Sick Sinus Syndrome Combined With Yang Deficiency And Blood-Stasis Syndrome

Objective:(1)Sick sinus improved interventional sodium hydroxide produced in rats sick sinus syndrome animal model.(2) According to traditional Chinese medicine syndrome characteristics,Compare changes in heart rate, weight, respiratory rate, body temperature, blood rheology indicators with Yang rat model of stasis sick sinus syndrome and Yang stasis rat model of non-disease, Judgment of sick sinus syndrome in rats model of Yang deficiency and blood stasis syndromes property.Methods:(1) the establishment of animal model of sick sinus syndrome in rats: anesthesia in rats after connecting heart motor, use an ophthalmic scissors cut a longitudinal skin incision with the right edge of the sternum at the second intercostal, About0.1cm, the homemade electrode catheter along the mouth into the chest, Determine the site of administration according to lead ECG waveform,homemade electrode catheter by the microinjection of10%sodium hydroxide solution.Measurements before modeling, after modeling5min,10min,30min,120min heart rate to determine the effect of modeling.(2)Contrast with Yang rat model of stasis sick sinus syndrome and Yang stasis rat model of non-disease:Daily with ice cold compresses the disease group Yang deficiency and blood stasis in rats on the chest area and put rats at4℃freezer4h,7consecutive days,And in the first five days of subcutaneous multi-point injection of epinephrine (1μ g/kg weight)。The Yang and blood stasis group sick sinus syndrome use the rat model which successfully produced by the above method,and the Control group without treatment.Comparison of the three groups of Chinese medicine Yang deficiency and blood stasis syndrome indicators which including weight, respiratory rate, body temperature, heart rate, blood rheology and so on.Judgment in the rat model of sick sinus syndrome of TCM syndrome properties.Results:(1)Improved sodium intervention were successfully manufactured rat model of sick sinus syndrome,and the heart rate decreased by more than30%,and the mortality rate of8%.(2)Comparison of the two groups of rats before and after modeling, weight,respiratory rate, body temperature, heart rate, blood rheology indexes have significant changes (P <0.01).Except the heart rate between two groups of rats (P <0.01) in index had no significant change (P>0.05).Conclusion:(1)The improved modeling methods was optimized the modeling process and improved the success rate of modeling.(2)the sick sinus syndrome rats model conforms to the Yang deficiency and blood stasis syndrome of traditional Chinese medicine syndrome properties,which produced by the improved sodium hydroxide Intervention.