The Effects Of Pingchuan I Hao On Pcna In Lung Tissue Of Rats With Acut Exacerbation Of Bronchial Asthma

Objective:To observe influence of Pingchuan I hao on the airway remodeling and the PCNA expression in the lung tissue, and dicuss the mechanism of Pingchuan I hao on asthma.Methods:60wistar rats were divided randomly into the normal control group, the model group, the dexamathasone group, the TCM low-dose group, the TCM middle-dose group and the TCM high-dose group. The asthmatic rat model were establish by the method of OVA and aluminum hydroxide.24hours after the last stimulation the rats were put to death. The left lung were took in sagittal plane based, and the airway changes were observed under the light microscope by pathological-section and HE staining according to the conventional method. The left lung were fixed in4%paraformaldehyde. The PCNA protein expressed in the lung tissue were detected by immunohistochemical method (PV two step method).Results:The lung tissue in the normal control group observed by HE staining under microscope showed that there were no inflammation cells around bronchi and vessels, no increasing in bronchial wall or area and no airway remodeling. The results in the model group showed that bronchial epithelial was incomplete with increased goblet cells, airway epithelial cell shedding, submucosal edema and increased mucus glands; there were thickened airway smooth muscle, inflammatory cells (mainly lymphocytes and eosinophils) infiltration in airway wall, airway and perivascular, hypertrophied bronchial smooth muscle cells, increased bronchial wall area, mucus blocking and bionchi stenosis. Every treatment group:the degree of proliferation of bronchial mucous membrane was unequal with an excessive mucous secretion; there were lymphocytes and eosinophils infiltration at different levels with edema in some of bronchi and the edema in model group was not more serious. Comparion among treatment groups:the pathological changes in middle-dose and high-dose groups were similar to the dexadecadrol group, while the changes in the low-dose group were more serious than the above three groups.After the image analysis software processing model rats found a significant increase in airway wall thickness of airway smooth muscle eell proliferation significantly. The thickness of airway smooth muscle, airway wall thickness, bronchial smooth muscle cells, were observed after comparing standardized find the respective index model group were significantly higher (P<0.05).The expression of PCNA in the normal control group was negative, while the model group strong positive being more powerful than the normal control group (P<0.05). The expression of PCNA in all the treatment groups were all less than that of the model group. The expression of PCNA in both of the middle-dose and high-dose groups were almost the same to the dexadecadrol group (P>0.05), while the expression in the above three groups were all less than the low-dose group (P<0.05). There was no difference between the middle-dose and the high-dose group (P>0.05).Conclusion:1.Pingchuan I hao can restrain the airway remodeling in asthma rats.2.Pingchuan I hao can reduce PCNA expression in lung tissue of asthma rats.