| Objective:To discuss the mechanism of "Pingchuan I hao" on bronchial asthma by observing its effects on ERK expression and activation in lung tissue of asthmatic rats.Methods:60heathy wistar rats were randomly divided into6groups:the normal control group, the asthma model group, the dexadecadrol group, the TCM low-dose group, the TCM middle-dose group and the TCM high-dose group. Establish the asthmatic rat model by using the method of OVA and aluminum hydroxide. The rats were put to death24hours after the last stimulation. The left lung were took in sagittal plane based, and the pathological changes were observed by pathological section and HE staining according to the conventional method. The ERK1/2in the left lung were detected by immunohistochemical method (PV two step method) after the left lung were fixed in4%paraformaldehyde. The images were analysed by image-analysis system. Light density of positive cells were analysed by the Meta Morph software, with3pictures at each point in time and5fields of vision in each section.Results:The lung tissue of rats in the normal control group observed by HE staining showed that there were no inflammatory cells around bronchi and vessels, no bronchial wall or area increased and no airway remodeling phenomenon. The lung tissue in the asthma model group showed that bronchial epithelial was incomplete with increased goblet cells, airway epithelial cell shedding, submucosal edema and increased mucus glands;there were thickened airway smooth muscle, inflammatory cells (mainly lymphocytes and eosinophils) infiltration in airway wall, airway and perivascular, hypertrophied bronchial smooth muscle cells, increased bronchial wall area with the bionchi blocked by mucus and bionchi stenosis. Every treatment group:the degree of bronchial mucous membrane proliferation was unequal with an excessive mucous secretion; there were lymphocytes and eosinophils infiltration at different levels with edema in some of bronchi and the edema in model group was not more serious. Comparion among treatment groups:the pathological changes in middle-dose and high-dose groups were similar to the dexadecadrol group, while the changes in the low-dose group were more serious than the above three groups. After the image analysis software’processing model rats found a significant increase in airway wall thickness of airway smooth muscle cell proliferation significantly. The thickness of airway smooth muscle, airway wall thickness, bronchial smooth muscle cells, were observed after comparing standardized find the respective index model group were significantly higher.ERK expression on the bronchiole smooth muscle was positive in the model and each treatment groups. The optical density of ERK on bronchial smooth muscle in the above groups were all higher than the normal control group. The expression in the model group was much higher than every treatment group (P<0.05). There were no differences in the expression of ERK among the middle-dose and high-dose groups and the dexadecadrol group (P>0.05). However, the expression in the low-dose group was better than the other three groups (P<0.05).The content of p-ERKl/2in the model group was obviously higher than the normal control group and the dexadecadrol group. The contents of p-ERK1/2in both of the middle-dose and high-dose groups were almost the same to the dexadecadrol group, while the contents in the above three groups were all lower than the low-dose group. There was no difference between the middle-dose and the high-dose group.Conclusion:1.Pingchuan I hao can restrain the airway remodeling in asthma rats.2.Pingchuan I hao can restrain the ERK expression and activation on the bionchial smooth muscle. |
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