DNA Ploidy Analysis Application Value In Cervical Lesions

Objective: This research mainly through the detection of cervical cancerand precancerous tissue DNA times body state to explore different times in theDNA of cells in cervical lesions occur in the development of the biologicalcharacteristics, and then forecasts the development trend of cervical lesion,provide the basis for clinical diagnosis and treatment.Method: From Mar.2013to Feb.2014，Collection specimens from385cases of liquid based cytology of outpatient service of department ofgynaecology and hospitalized patients underwent DNA times body analysis inthe second hospital of Hebei Medical University, as the gold standard ofdiagnosis by histopathological results. Clinical manifestations:leucorrheaincrease(No clinical symptoms because of physical examination found that thecervical lesions)、Bleeding after intercourse、Postmenopausal bleeding、Irregular vaginal bleeding、Abnormal drainage、Abdominal pain、Cervicalerosion.Results: Collection specimens from385cases of liquid based cytologyunderwent DNA times body analysis in the second hospital of Hebei MedicalUniversity. Including: liquid based cytology specimens of310cases ofASCUS，LSIL specimen of73cases，Hospitalized patients final pathologicresult is2cases of adenocarcinoma of liquid based cytology specimens.383cases of ASCUS and LSIL specimens diagnosed liquid based cytology werecollected to histopathological results， more than CIN lesions are141cases(36.81%).71cases with cervical intraepit-helial neoplasia(CIN) I (50.35%)，20cases with CINⅡ (14.18%)，36cases with CINIII(25.53%)and14caseswith cervical invasive cancer(9.93%)were confirmed by colposcopy.Totally233cases were observed with DNA heteroploid，positive rate of60.84%. and150were not observed with DNA heteroploid.DNA heteroploid cell number of1-2is83cases, pathology results confirmed the high grade cervical lesions in 7cases, the positive rate was8.43%. DNA heteroploid cell number of3-9is80cases, the high grade cervical lesions in17cases, the positive rate was21.25%.DNA heteroploid cell number of≥10is70cases, the high gradecervical lesions in44cases, the positive rate was62.68%. Multiplecomparisons were statistically significant. When DNA heteroploid negativeand more than or equal to three ploid were used to predict CINⅡ or moresevere cervical diseases．the sensitivity，specificity, positive predictive valuesand negative predictive values were97.14%and89.71%,47.28%and46.06%,98.67%and91.57%, respectively. cases of DNA aneuploid cell negative andpositive for the number of cases of cervical lesion comparative difference wasstatistically significant (χ2=118.823, P<0.01).The test results of211cases of HPV in310ASCUS patients followed up,in which high-risk HPV positive was152cases,59cases of patients withhigh-risk type HPV negative. In high-risk HPV positive group:67cases ofDNA aneuploid cells were negative, pathological results of1cases of CINIII.85cases of DNA aneuploid cells were positive, the pathological resultsshowed that13cases of CINII,15cases of CINIII,9cases of cervical cancer.In the detection of high-risk HPV negative group:26cases of DNA aneuploidcells are negative, the pathological results showed that1cases of CINIII.33cases of DNA aneuploid cells were positive, the pathological results showedthat5cases of CINII,4cases of CINIII. In high-risk HPV positive group,pathologic DNA aneuploid cell negative, positive in high-grade cervicallesions were significant difference (χ2=35.311P<0.01). In the high-risk typeHPV negative group, pathologic DNA aneuploid cell negative, positive inhigh-grade cervical lesions were significant difference (χ2=4.128P<0.05).Conclusion: DNA quantitative analysis of cells as a kind of effectivemethod has been applied to study the pathological and clinical diagnosisanalysis in exfoliated cervical. Clinical pathology doctors can make relativelyobjective for each cell nucleus DNA content, less affected by cells themselvesmetamorphosis, in clinical detection has higher sensitivity and negativepredictive value. DNA ploidy analyzer of standardization has higher quality for benign and malignant tumors, which provides objective and accurateanalysis results for clinical diagnosis.