Study Of Buyanghaiwu Dcoction On Expression Of ENOS And INOS In Caveolin-1Knockout Mice

Objective:To exPlore the effect of Buyanghuanwu Decoction on eNOS and iNOS in rats after focal cerebral ischemiaMethods:Rats were randomly divided into following grouP including normal grouP, shame-oPerated grouP and Buyanghuanwu decoction grouP. Focal cerebral ischemic model was reProduced by middle cerebral artery occlusion. Animals were killed at1,3and7day Point after oPeration. The neurological severity score were be observe and eNOS and iNOS of cerebral cortex were measured by immunohistochemical technology. And observe the effects of Buyanghuanwu decoction.Result:(1) The neurological severity score of caveolin-1knockout rats were reduce after middle cerebral artery occlusion, but treatment of Buyanghuanwu decoction, it is increase. (2) the eNOS of cerebral cortex were reduce, the iNOS were increased after cerebral artery occlusion of caveolin-1knockout rats. But we have a oPPosite result after treatment of Buyanghuanwu decoction.Conclusion:(1) Knockout of caveolin-1can aggravate cerebra ischemia rats dysfunction and decrease exPression of eNOS and increase exPression of iNOS.(2) Buyanghuanwu decoction increase exPression of eNOS and decrease exPression of iNOS. And can Promote the nerve function.