| Objective: To establish the model of type2diabetic rats and undergogastric bypass surgery,through observing the changes of the blood-glucoseand the NPY level in the hypothalamus after operation,we research therelationship of the NPY with type2diabetes,and alteration of the gastricbypass surgical treatment,to provide a theoretical basis for the study ofRYGB in the treatment of type2diabetes mellitus.Method:(1)8-weeks-age male SD rats80, were randomly divided intothe normal food diet group (n=20) and the high-fat diet group (n=60), to befed high fat diet and normal diet10weeks respectively,the high-fat diet groupwere given streptozotocin (STZ)(30mg/kg) by intraperitoneal injection toestablish the model of T2DM rats.(2)The T2DM rats were randomly dividedinto no operation group(NO,n=10),shame operation group(SO,n=10),Roux-en-Y Gastric Bypass group(RYGB, n=10),ten normal diet rats were alsorandomly selected as normal control group(NC,n=10).(3)SO group andRYGB group after fed with high fat diet, the other groups according tooriginal respective feed to keep feeding, until the end of the experiment.(4)rats from the preoperative and postoperative fasting body weight weekly,8weeks after the surgery.(5)The FBG, FINS,TG, TC and HOMA-IR of rats ineach group were determined in preoperative4weeks and postoperative8weeks.(6)put the rats in each groups to death,and apply Western Blot andRT PCR quantitative determined the expression level of NPY in thehypothalamus. Result:1. The comparison of weight between high-fat group with normal dietgroup in the modeling process.No statistically significant differences between the two groups of rats10weeks after initial body weight, body weight of rats in the two groups werestatistically significant,and the P<0.01.2. The comparison of plasma NPY level in the High fat group and thenormal group rats after modeling successfullyTwo groups of rats after modeling successfully,comparison of thedetermination of plasma NPY level,the difference was statistically significant,P<0.01.3. The comparison of body weight of rats in four groups before and afterthe operationNo significant differences preoperative weight in RYGB group, NOgroup, SO group, three groups of rats, but there was significant differencewith NC group, P<0.01.10weeks after operation,no statistical significanceweight difference between NO group and SO group rats, P>0.05; no statisticalsignificance, weight difference between NC group and RYGB group rats, P>0.05; there was significant difference between the other groups of body weight,P<0.01.8weeks after operation in four groups of rats body weight andpreoperative self paired comparison, the differences were significant, P<0.01.4. The comparison of plasma NPY level in four groups before and afterthe operationThere was no significant difference of plasma NPY level in RYGB group,NO group, SO group, rats in the three groups before operation, but both hadsignificant difference with NC group, P<0.01.10weeks after operation, nosignificant difference between N0group and SO group, the levels of plasma NPY P>0.05; no significant difference between NC group and RYGB group,the levels of plasma NPY P>0.05; there was significant difference betweenthe level of NPY in plasma of rats in other groups, P<0.01. The level of NPYin plasma and in8weeks after operation in the four groups before its pairedcomparison, the differences were significant, P<0.05.5. The comparison of FBG in four groups before and after the operationComparison of plasma insulin levels in rats of the three groupspreoperative RYGB, NO, SO, no significant difference, P>0.05, the threegroup and NC group were significant difference, P<0.01. Comparison ofplasma insulin levels after operation in group NO, SO, RYGB, NC, there wasno significant difference between group, P>0.05, the difference between thegroups were statistically, among them RYGB, NC group, P<0.05RYGB, SOgroup, the rest of the P<0.01.Plasma insulin level before operation,postoperative self paired comparison, there are statistically significantdifferences in P<0.01, RYGB group, the other groups were not statisticallysignificant, P>0.05.6. The comparison of FINS in four groups before and after the operationThere was no significant difference of plasma insulin levels in RYGBgroup, NO group, SO group, rats in the three groups before operation, butboth had significant difference with NC group, P<0.01. After8weeks, nosignificant, difference between NC group and RYGB group rats plasmainsulin level P>0.05; no significant, difference between NO group and SOgroup rats plasma insulin level in P>0.05; in the other groups werestatistically significant, P<0.05. Four groups of rats plasma insulin levels ofeach before surgery, paired comparison, there was no significant difference ingroup NO, P>0.05; NC group, RYGB group and SO group were the differences, P<0.05.7. The comparison of TC in four groups before and after the operationRYGB group, NO group, SO group, the three groups of rats TC levelbefore operation, there was no significant difference in P>0.05, comparedwith NC group, there was statistically difference, P<0.05. The comparisonbetween the8weeks of NO group and SO group TC after operation, therewas no significant difference, P>0.05; comparison between RYGB group andNC group, the levels of TC, there was no significant difference among othergroups, P>0.05, TC level has no difference. The TG level of each pairedcomparison of rats in the four groups before and after operation, NC groupand SO group, P>0.05, and differences between RYGB group and NO grouphad statistical significance, P<0.05.8. The comparison of TG in four groups before and after the operationComparison of plasma triglyceride levels in rats of three groupspreoperative RYGB, NO, SO, no significant difference, P>0.05, the threegroup and NC group were significant difference, P<0.01. Triglyceride levels8weeks before surgery, the paired comparison, there are statistically significantdifferences in P<0.01, RYGB group, the other groups were not statisticallysignificant, P>0.05.9. The comparison of Four groups of rats at hypothalamic NPYexpression Western Blot results in four groups before and after the operationin the hypothalamic.8weeks after operation between NO group and SO group, the expressionof NPY in hypothalamus of no significant difference, P>0.05; there wassignificant difference among other groups of NPY expression, P<0.01.10. The comparison of NPY RT PCR mRNA expression in four groups before and after the operation in the hypothalamic.8weeks of hypothalamic NPY mRNA levels after operation werecompared, no significant differences between groups, group SO and NOP>0.05, among other groups showed no significant differences, P<0.01.Conclusion:1. NPY level in type2diabetic rat plasma was significantly higher thanthat of normal rats, the effect of glucose and lipid metabolism in NPY, closelyrelated to the occurrence and development of obesity and T2DM.2. Gastric bypass surgery can significantly reduce the risk of type2diabetes rat body weight and fasting blood glucose, plasma insulin levels, andimprove insulin resistance and decrease the plasma triglyceride andcholesterol levels, confirmed gastric bypass operation on treatment of theexact effect in type2diabetic rats.3. After gastric bypass surgery plasma, hypothalamus in type2diabeticrats NPY expression levels were significantly reduced, that this may begastric bypass to reduce weight, improve the metabolic syndrome, one of themechanisms in the treatment of T2DM. |
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