The Study Of Thermo Metabolic Features In Infertility With Yang-deficiency And The Influence Of Yougui Pill On ATP And NO In Model Female Rats With Yang-deficiency

ObjectiveTo explore the infrared thermal imaging features of infertility with Yang-deficiency, and the influence of Yougui Pill (YGP) on model rats with Yang-deficiency.Methods1.Infertility patients and healthy female for routine examinations were collected in the Second Affiliated Hospital of Chengdu university of Traditional Chinese Medicine (TCM) from August,2011to December,2012. The questionnaires of Classification and Determination of Constitution in TCM were filled. The voluntary participants without history of pelvic inflammatory disease were scanned by infrared thermal imaging. Consequently the calorific values of six body regions were measure including forehead, feet, Du Channel, Shenque point, lower abdomen and uterus. Data were analyzed by SPSS17.0.2.(1)16female ICR mice were randomly assigned into3groups, including the control group (N=6), model group (N=5) and YGP group (N=5). Rats in control group were fed with normal saline, while other two group with hydroxyurea suspension liquid for19days. The female mice were mated with male mice at8th day and vaginal smear were observed. The infertile rats in YGP group were fed with high concentration of YGP decoction at13th day for another7days. Then the rest infertile mice in three groups were continued to be mated with vaginal smear, and pregnancy rate were calculated.(2)66female ICR mice were randomly assigned into3groups, including control group (N=22), model group (N=22), YGP group (N=22), and were fed as above method for15days. Rats in YGP group were fed with high concentrations of YGP decoction at9th day for7days besides hydroxyurea. The rectal temperature was measured every day and body weight was measured every other day. On the16th days, all mice were sacrificed. The oocytes of10mice were randomly taken in each group, and nude oocytes were soaked in cell lysis solution. Then, the ovaries of rest12mice were taken, the tissues were homogenated, centrifuged and the supernatant were obtained. Finally ATP, ATPS, NO, NOS and total protein content were tested. Data were analyzed by the software of SPSS17.0.Results1.355valid questionnaires were collected, including188of the infertility and167of health. The proportion of Yang-deficiency constitution in infertility group (29.26%) was higher than that in healthy group (19.17%)(P<0.05). There were142cases being completely scanned by infrared thermal imaging, including97cases of infertility with Yang-deficiency,16cases of infertility without Yang-deficiency, and29cases of the healthy. Calorific values of Du channel, Shenque point, lower abdomen and uterus in infertility group with Yang-deficiency, were significantly lower than those in infertility without Yang-deficiency group and healthy group (P <0.05).2.After the model was established successfully, estrus cycle of mice were irregular or disappear. The weight, anal temperature and conception rate of Yang-deficiency model mice were lower than those in the control group (P<0.05), The mice weight and anal temperature in YGP group gradually increased after fed with YGP decoction7days later, and the conception rate was higher than that in model group (P<0.05).3.The oocyte amount in model group was less than that in YGP group (P<0.01). The ovary index was lower than that in the control group and YGP group (P<0.05).4.The absolute content of follicular ATP, ATPS, NO, NOS and the ovarian absolute content of ATP, ATPS in model group were lower than those in the control group and YGP group (P<0.05). The absolute content of ovarian NO in model group was higher than those in the control group and YGP group (P<0.05). The absolute content of follicular and ovarian ATPS in YGP group was lower than that in the control group (P<0.05). There was no significant difference among ovarian NOS in three groups (P>0.05).Conclusion1.The infrared thermal imaging of infertility with Yang-deficiency is featured as low calorific value of body region including Du channel, Shenque point, lower abdomen uterus, etc.2.YGP can improve Yang deficient symptoms and increase the anal temperature and pregnancy rate of mice with Yang-deficiency.3.YGP have the positive action on ATP and NO metabolism in follicular and ovarian tissues of infertile mice with Yang-deficiency, and the mechanism is related to the change of the contents and activities of NOS and ATPS.