The Effect Of Platelet-rich Fibrin On Periodontal Ligament Cells And Rat Osteoblast In Vitro

ObjectiveThe objective of this study was to analyze the effects of Choukroun’s PRF(platelet-rich fibrin) on human periodontal ligament cells (PDLCs) and ratosteoblast in vitro, to provide a theoretical basis on periodontal tissueregeneration and repair materials.MethodsThis article through the Choukroun’s PRF carrying into the subculture ofPDLCs and rat osteoblast, without PRF of calf serum-free culture solution iscontrol group. According to each culture times, cell proliferation was detected byMTT method, differentiation was determined by measuring alkalinephosphatase (ALP) activity, the total protein content of the periodontal ligamentcells was determinated by coomasie brilliant blue staining, OPG and RANKLgene expression were quantified by western-blot. Statistical analysis usingSPSS17.0package.Results1. At the3,7,14,21days of the experiment, MTT show that PRF generateda significant stimulation of the PDLCs and the osteoblast proliferation, the valueof A statistically significant (P <0.05). And the data of the experimental,respectively greater than the control group.2. PRF generated a significant stimulation of the PDLCs and the osteoblast differentiation throughout the experimental period compared with the controlgroup, the difference was statistically significant (P <0.05).3. Determination of total protein content of the results, the statisticalanalysis show that periodontal ligament cells in all experimental time,experimental group data were higher and statistically significant(P <0.05).4. Western blot show that PRF obviously increased the gene expression ofOPG, especially in experiments1and3days. However, there was no significantchange in RANKL expression.ConclusionsChoukroun’s PRF stimulated human PDLCs and rat osteoblast proliferationand differentiation, so as in the protein synthesis of PDLCs, it also promoteosteoblast gene expression of OPG in vitro that regulated the balance betweenosteoblast and osteoclast by affecting the gene expression of OPG and RANKL.Suggesting that Choukroun’s PRF is possibly the promising growth factor in theregion of periodontal tissue regeneration.