Study On The Mechanisms And Regularity Of The Inactivation Of Adenovirus41by Chlorine Dioxide In Water

Objective:To evaluate the role of the destruction of viral DNA and capsid protein in the inactivation of Adenovirus41by Chlorine dioxide,so as to provide the basis for elucidating the inactivation and the molecular mechanism, and lay the theoretical and technical foundation for the disinfection of enterovirus in water. Methods:AdV4l,E. coli25922, bacteriopHage MS2, polio virus type1(PVl)were used to the different initial concentrations of chlorine dioxide. Using UV-isible spectropHotometric to determine the concentration of chlorine dioxide during the experiment, a classic onethe kinetic model Ct=C0e-kt to fit the the disinfectant decay experiment.Cell culture was applied to determine viral infectivity at different Chlorine dioxide dosages for different contact times. Filter method and double plate methods were used to detect the remaining E. coli and bacteriopHage MS2.Using two inactivated dynamics models of Chick-Watson and Efficiency factor Hom to evaluate the disinfecting effect of chlorine dioxide on microorganisms.Long-overlapping polymerase chain reaction (PCR) was used to detect the damage of viral genome. The detection of viral capsid protein was deteced using enzyme-linked immunosorbent assay (ELISA). Results:Chlorine dioxide concentration attenuation law was in accordance with a dynamic model Q=Coe-ktin various microbial contamination in water samples. The inactivation rates were increased with Ct values in Chick-Watson model. With the Efficiency factor Horn (EFH) model,the longer the inactivation time,the better the inactivation effect Different regions of AdV41viral genome had different chlorine dioxide-resistance. The damage of the1-2081nt of the genomic5regions was in coincidence with the inactivation of AdV41infectivity.Then dividing the region further, the area of1015-1402nt was first injured.1015-1402nt of AdV41was associated with the viral infection.The ELISA test AdV41capsid protein expression after disinfection. Damage of AdV41antigen was after the DNA damage.And the injury of viral antigen presents dose-effect relationship. Conclusion:Chlorine dioxide concentration attenuation law was in accordance with a dynamic model Ct=Coe-y Chlorine dioxide accords with the model of the the inactivated dynamics model of Chick-Watson and Efficiency factor Hom.Rsistance to chlorine dioxide from weak to strong orders of E.coli25922, bacteriophage MS2, AdV41, PV1. AdV41genome of1015-1402nt regional is the weakest resistance to chlorine dioxide, and associated with the viral infection. The enteric adenovirus nucleic acid damage prior to the viral capsid protein damage; damage of the capsid protein and viral infections disappeared was irrelevant.