Research On The Relationship Between Thrombomodulin Gene Polymorphism And Cerebral Infarction

Objective:To study the relationship between thrombomodulin gene (rs1042579、rs1042580、rs3746726、rs3746728) polymorphism and the cerebral infarction.Material and Methods:351patients with cerebral infarction were selected.471age and sex-matched healthy individials were recruited as a control group. PCR amplification and Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) were used to detect genotype of Thrombomodulin genetic polymorphism. Finally we make statistical analysis of four locus genotypes、frequencies of allele and haplotype between case and control groups.Results:1. Four SNPs loci (rs1042579、rs1042580. rs3746726. rs3746728) were found in populations from Changsha, Hunan Han.2. There is no statistical difference between the frequencies of genotype and allele of four SNPs loci (rs1042579、rs1042580. rs3746726. rs3746728)(P>0.05, statistical methosd).3. Logistic regression analysis indicates that thrombomodulin gene rs1042580polymorphism is an possible genetic risk factor for hypertension.4.The SNPs loci (rs1042579、rs1042580. rs3746726. rs3746728) is located in the same haplotype block (0<D’<1). There are six haplotypes (CAGT. CATC. CGGT、CGTC、TAGT、TATC) and the frequency of each haplotype in the case and control groups showed no statistically significant (P>0.05, statistical methosd)Conclusion:1. Thrombomodulin gene (rs1042579, rs1042580, rs3746726, rs3746728) SNPs loci polymorphism may not be the genetic risk factor for cerebral infarction in Changsha, Hunan Han population.2. Thrombomodulin gene rs1042580locus with A allele is an possible genetic risk factor of hypertension in Changsha, Hunan Han population.3. It is a first report that there are six haplotypes CAGT、CATC、CGGT、 CGTC、TAGT、TATC existing in rs1042579、rs1042580、rs3746726. rs3746728SNPs of thrombomodulin gene and they are all unrelated to the cerebral infarction.