The Relationship Between The Thrombomodulin Promoter-33G/A Mutation And Cerebral Infarction

Objective:Thrombomodulin (TM) is an endothelial glycoprotein which can activate protein c and plays an important role in the coagulation. Recently, conflicting data have showed about the effect of-33G/A polymorphism of TM promoter region gene on cardio-cerebrovascular disease. Then, we investigated the association of the-33G/A mutation of thrombomodulin gene and cerebral infarction in Chinese han people.Materials and methods:130 cerebral infarction cases and 120 controls admitted to the department of neurology at the First People's Hospital of Yunnan province between November 2009 and June 2010 were studied.They were Chinese Han. These cerebral infarction cases matched the diagnostic criteria (including medical history, physical examination and head CT or MRI imaging diagnosis) revised at the Forth National Symposium on cerebrovascular disease in 1995. Controls without cardio-cerebrovascular disease were recruited among individuals hospitalized at the same time, who were matched to cerebral infarction patients by sex and age. Clinical data were collected.The TM-33G/A polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP).Results:â‘¡In 130 patients with cerebral infarction,GG genotype was 99, GA genotype was 30, AA genotype was 1, and the G/A point mutation rate was 23.85%. In 120 cases of the control group, GG genotype was 106, GA genotype was 13, AA genotype was 1, and the G/A point mutation rate was 11.67%.There was significant difference between the two groups (P<0.05).â‘¡Multivariate logistic regression analysis revealed that, in addition to history of smoking and hypertension, increased low-density lipoprotein cholesterol, increased fasting glucose and TM-33G/A polymorphism were also independent risk factors for cerebral infarction (OR=2.175,95%CI:1.049-4.511, P=0.037)Conclusions:â‘ The results suggest that history of smoking, hypertension and diabetes, increased low-density lipoprotein cholesterol, increased fasting glucose and reduced high-density lipoprotein cholesterol are risk factors for cerebral infarction.â‘¡There was a significant association between the thrombomodulin -33G/A polymorphism and cerebral infarction in yunnan Chinese han people, the thrombomodulin-33G/A polymorphism may be a risk factor for cerebral infarction.