Study On The Expression Level Of Ang-1, Ang-2, Annexina2Gene In Peripheral Blood And TCM Syndrome Of Type2Diabetic Nephropathy

Objective: Study on the expression level of Ang-1, Ang-2, AnnexinA2gene inperipheral blood and TCM syndrome of type2diabetic nephropathy.Methods:According to the standard of diagnosis and elimination of internationalunified, included in the case. A total of108patients included in the collection ofperipheral blood samples of patients, including40cases of type2diabetic nephropathyand38cases of type2diabetic patients and30cases of healthy people. The experimentbased on108blood samples for extraction of RNA and inverted into cDNA，Throughthe analysis of PCR for Ang-1, Ang-2, AnnexinA2expression. According to the designof the TCM syndrome questionnaire, collecting patients with general information,symptoms, tongue, pulse etc. For TCM syndrome investigation to the researchers anddialectical analysis, Expression of3genes of interest in the study of different TCMsyndromes.Results: All patients included in, The statistical analysis has no statistical significancein terms of gender, age, BMI, duration, complications, associated with other diseases indepartment of internal medicine, clinical comparable. Qi-Yin deficiency of Liver andkidney(QYDLK) is more than Yang deficiency of spleen and kidney(YDSK), bloodstasis and syndrome of damp-heat syndrome of QYDLK is more than YDSK, QYDLKand two clamping blood stasis syndrome and damp-heat syndrome more than YDSK.The incidence of diabetic microangiopathy and gangrene, hypertension, dyslipidemia,coronary heart disease incidence rate. Expression of PCR using fluorescencequantitative detection method for3target genes in a sample of108cases andapplication of statistical analysis, Results showed that3genes expressed in the DNgroup, DM group, healthy control group, The average expression of DN group washigher than that in DM group and the healthy control group,P<0.05, The difference was statistically significant. Expression of3genes in40patients with DN syndrome in twoprincipal syndromes are different, The average expression of YDSK is higher than thatof QYDLK,by T test,P<0.05, The difference was statistically significant.3geneexpression in two and card folder, the difference is not statistically significant.Conclusions:According to this study, Liver and kidney yin deficiency and yangdeficiency of spleen and kidney two in type2diabetic nephropathy is the most, bloodstasis and syndrome of damp-heat syndrome of QYDLK is more than YDSK, QYDLKand two clamping blood stasis syndrome and damp-heat syndrome more than YDSK.The incidence of diabetic microangiopathy and gangrene, hypertension, dyslipidemia,coronary heart disease incidence rate.3genes expressed in the DN group, DM group,healthy control group, The average expression of DN group was higher than that in DMgroup and the healthy control group; Expression of3genes in the TCM syndromes oftwo main syndrome, The average expression of YDSK is higher than that of QYDLK;3gene expression in two and card folder, the difference is not statistically significant.