Clinical Significance And Serum Level Of Glyoxalase I In Type2Diabetic Patients With Nephropathy

[Objective]To observe the serum level of glyoxalase I (GLOI), and the correlation to advancedglycation end products（AGEs）and secretory receptor for advanced glycation endproducts（sRAGE）, to explore the clinical significance of GLOI in the diabeticnephropathy (DN).[Methods]Select106type2diabetes patients who were hospitalized in the department ofendocrinology, Fujian Medical University Affiliated Union Hospital, from March2012to March2013as the diabetes group. Separate these patients into3groups:normal albuminuria group, microalbuminuria group and massive proteinuria group. Inaddition, choose36healthy people in the same period as the normal group forcompare. Measure various indicators of each group, which including height, weight,and determine serum triglyceride （TG）、serum total cholesterol(TC)、serumhigh-density lipoprotein cholesterol(HDL-C)、 serum low-density lipoproteincholesterol(LDL-C)、fasting blood glucose, glycosylated hemoglobin（HbAlc）,GLOI, sRAGE、 AGEs, morning urine creatinine and morning urine albumin.Calculate their body mass index (BMI), urinary albumin creatinine ratio (ACR), andcompare the different indicators between each group of patients and analyse theirrelationship with each other and use relevance comparison method to analyseinfluencefactors.[Results]1. Clinical data comparisonThe indicators of age, BMI, systolic pressure, diastolic pressure, TG, LDL-C, fastingglucose are significance higher than normal group, and HDL-C is lower than normalgroup.The difference have statistical significance (P <0.05). TC is higher than normalgroup with no statistical significance（P>0.05）. Those indicators between diabetesgroups show no statistical significance（P>0.05）.2. Compare GLOI、sRAGE、AGEsIn the sequence of the normal group, normal albuminuria group、microalbuminuriagroup and massive proteinuria group., we can see that the level of GLOI（P<0.05） and sRAGE（P <0.001）are declined, the level of AGEs is increased（P<0.001），and the difference have statistical significance. However, compare microalbuminuriagroup to massive proteinuria group, those3indicators be mentioned above have nostatistical significance.3. Relevance comparisonGLOI has negative correlation with systolic pressure、 TG、 LDL-C、 fastingglucose、AGEs, but has positive correlation with urine creatinine（P<0.05）. AGEshas positive correlation with systolic pressure、ACR、fasting glucose、HbA1C，and has negative correlation with GLOI（P<0.05）. sRAGE has negative correlationwith age、BMI、systolic pressure、TG、fasting glucose and has positive correlationwith GLOI、HDL-L（P<0.05）.4. Multiple Linear Regression comparisonThe influence factors of GLOI are course of disease and urine creatinine. Theinfluence factors of AGEs are course of disease and HbA1C. The influence factor ofsRAGE is relates to course of disease.[Conclusion]1. Compare to normal group, the levels of GLOI and sRAGE are both declined, thelevel of AGEs is increased in diabete groups, which shows that hyperglycemia hasimpact to the level of GLOI and GLOI has correlation with AGEs、sRAGE.2. The level of GLOI in the type2diabetic nephropathy is declined in the early stage,and is affected by course of disease. It suggests that GLOI may be involved withdiabetic nephropathy, and its level can be regard as an indicator to the renalmicrovascular lesions3. GLOI has negative correlation with systolic pressure、 TG、 LDL-C、 fastingglucose、 AGEs, which suggest that the level of GLOI can be influenced byhypertension, hyperlipidemia, hyperglycemia, AGEs.