The Primary Research Of The Relation Between VIP Expression And Macrophage Polarization In Gastric Carcinoma Tissues

Objective：To research the relation between the VIP expression and macrophagepolarization in gastric carcinoma tissues.Method：Fifty-eight patients who received surgery for gastric cancer from March2011toDecember2012at the First Affiliated Hospital of Nanchang University were enrolledin our study. Gastric carcinoma tissue, normal tissue peripheral to the carcinoma, andpatient information were collected from each patient. Among the patients studied,43were male,15were female. The age of patients in this study ranged from25to85,with an average of57.22±11.32. Thirty-three patients were below59years old, theother25were above60years old. Five tumor tissues located at fundus（8.6%）,20tumor tissues at the body of the stomach（34.5%）, and33tumor tissues at the pyloricantrum（56.9%）. Forty-four cases had lymph node metastasis,14cases had no lymphnode metastasis. Thirty-three cases were highly or moderately differentiated,25caseswere poorly differentiated. According to the TNM stage,25cases were in TNM stageI to II, and33cases were in TNM stage III to VI. Immunohistochemical method wasused to detect the expression of VIP, IL-10, CD68, and IL-12proteins.Results:1. The positive expression rates of VIP in gastric carcinoma tissue (84.5%) wassignificantly higher than that in normal peripheral tissue(67.2%)（P<0.05）. Theexpression intensity of VIP in gastric carcinoma was significantly higher than that innormal peripheral tissue (P<0.01).The VIP expression intensity in the patients withpoorly differentiated degree, lymph node metastasis, or TNM III to IV, wassignificantly higher than that in patients with well-moderately differentiated degree,no lymphnode metastasis, or TNM I to II respectively (P<0.05). However, the VIPexpression intensity had no difference with respect to the age, sex, or cancer locationwithin the body (P>0.05). 2. The positive expression rates of CD68in the inflammatory cells of gastriccarcinoma tissues (87.9%) was significantly higher than that in normal peripheraltissues (56.9%)(P<0.01). The expression intensity of CD68in the inflammatory cellsof gastric carcinoma significantly was higher than that in normal peripheral tissue(P<0.01). The CD68expression intensity in the atients with poorly differentiateddegree, was significantly higher than that in patients with well-moderatelydifferentiated degree (P<0.05).However, the CD68expression intensity in theinflammatory cells of gastric carcinoma had no difference with respect to the age,sex,cancer location within the body, lymph node or different TNM（P>0.05）.3. The positive expression rates of IL-10in the inflammatory cells of gastriccarcinoma and adjacent normal mucosa beside carcinoma were65.5%and62.1%respectively. There were no significant difference between them (P>0.05)．Theexpression intensity of IL-10in the inflammatory cells of gastric carcinomasignificantly was higher than that in normal peripheral tissue (P<0.05).The IL-10expression intensity in the inflammatory cells of gastric carcinoma in the lymph nodemetastasis,or TNM III to IV,was significantly higher than that in patients with nolymphnode metastasis,or TNM I to II respectively (P<0.01). However, the IL-10expression intensity had no difference with respect to the age, sex, cancer locationwithin the body or differentiation groups (P>0.05).4. The positive expression rates of IL-12in the inflammatory cells of gastriccarcinoma and adjacent normal mucosa beside carcinoma were93.1％and93.1％respectively, with no difference between them (P>0.05)．The expression intensity ofIL-12in the inflammatory cells of gastric carcinoma significantly was lower than thatin normal peripheral tissue (P<0.01).The IL-12expression intensity in the atients withpoorly differentiated degree or TNM III to IV, was significantly lower than that inpatients with well-moderately differentiated degree or TNM I to II respectively(P<0.05). However, the IL-12expression intensity had no difference with respect tothe sex, age, cancer location within the body or lymph node (P>0.05).5. There was positive correlation between the expression intensity of VIP incarcinoma tissues and the expression intensity of CD68and IL-10in theinflammatory cells of gastric carcinoma tissues. There was negative correlation between the expression intensity of VIP in carcinoma tissues and the expressionintensity of IL-12in the inflammatory cells of gastric carcinoma tissues. There waspositive correlation between the expression intensity of CD68and the expressionintensity of IL-10in the inflammatory cells of gastric carcinoma tissues. There wasnegative correlation between the expression intensity of CD68and the expressionintensity of IL-12in the inflammatory cells of gastric carcinoma tissues.Conclusion:VIP may promote M2polarization of local macrophages in gastric carcinomatissues, which may contribute to gastric cancer cells to escape the host immuneresponse.