| Objective: To observe and analyze the impact of the basil polysaccharideson human ovarian cancer SK0V3cytoskeleton and SEMA4C expression, andreveal the mechanism of basil polysaccharide to inhibit the metastasisof ovarian cancer.Methods°: Human ovarian cancer SK0V3was cultured in37C,5%C02in vitro.Different concentrations of basil polysaccharide were used to treat thecells, cellular morphology of different groups were observered,microfilament and microtubules changes in the cells were observed byimmunofluorescence in each group, SEMA4C mRNA expression was detected byreal-time PCR in the differently treated cells, and SEMA4C proteinexpression were analyzed by immunocytochemistry.Results: Basil polysaccharide can change the microfilament andmicrotubules system of the SK0V3ovarian carcinoma cells, and basilpolysaccharide can inhibit SEMA4C mRNA and protein expression of theovarian cancer, in addition, the inhibition effect became more obviouswith the increase of drug concentration.Conclusion: Basil polysaccharide could change cytoskeleton system ofSK0V3and down-regulate the expression of SEMA4C, a lymph metastasis-related gene, which may be a new mechanism of basilpolysaccharide to inhibit lymph metastasis of ovarian cancer. |
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