The Expression Of Sema4C And Its Significance In Epithelial Ovarian Cancer

ObjectiveTo detect the expression of protein and its mRNA in human epithelial ovarian cancer and to analyze the relationship between Sema4C and the clinical pathology parameters of human epithelial ovarian cancer,such as the differentiation degree,clinical stage,pathology type,age of onset and ascites.And supply valued diagnosis and prognosis indicators for clinical workers.Further more,to detect the expression of Sema4C in cell of ovarian cancer and study the role of Sema4C in the growth and progression of ovarian cancer and future to interpret the possible molecular mechanisms.MethodsTissue:Real time Quantitative polymerase chain reaction was employed to detect the expression of Sema4C mRNA in the following tissues samples:74 patients with epithelial ovarian cancer,15 patients with borderline epithelial cancer,15 patients with benign epithelial tumor and 15 normal ovaries.Immunohistochemical staining of tissue microarrays was conducted to detect protein expression and locations of Sema4C protein in the tissue of 111 patients with epithelial ovarian cancer,30 patients with benign epithelial,30 patients with borderline tumor and 30 patients with normal ovaries.Cell:Real time Quantitative polymerase chain reaction and cell immunohistochemistry were employed to detect the expression of Sema4C mRNA in the cell of human ovarian cancer and human fibroblasts.Results1.Sema4C mRNA:The expression of Sema4C mRNA in the group of epithelial ovarian cancer was significant higher than that in benign,normal and borderline group(P＜0.05),and the expression of Sema4C mRNA was no significant difference among benign,normal and borderline group.Considering the clinical pathology parameters of human epithelial ovarian cancer(such as FIGO stage,differentiation degree,pathology type,age of onset and hydroperitoneum),the expression of Sema4C mRNA in the ovarian cancer group of stageⅢ-Ⅳand poorly differentiated was significant higher than that in stageⅠ-Ⅱand high or moderately differentiated group(P＜0.05),and the expression of Sema4C mRNA was not correlated with pathology type,age of onset and hydroperitoneum(P＞0.05).2.The expression of Sema4C protein:The Sema4C was mainly located in cytolymph and(or) cellular membrane through immunohistochemical outcome.The expression of Sema4C protein in the group of epithelial ovarian cancer was significant higher than that in borderline,benign and normal group(P＜0.05),and there was no significant difference among other three groups.The expression of Sema4C protein in the ovarian cancer group of advanced stage and poorly differentiated was significant higher than that in early stage and high differentiated group(P＜.05),and the expression of Sema4C protein was not correlated with pathology type,age of onset and ascites(P＞0.05).3.Among the 75 cases followed up,19 cases survive five years,the accumulative survival rate was 25.33%and the median survival time was 27 months.The 8 cases from 47 cases of positive expression of Sema4C survive five years,the accumulative survival rate was 17.02%and the median survival time was 19 months.The 11 cases from 28 cases of negative expression of Sema4C survive five years,the accumulative survival rate was 39.29%and the median survival time was 31 months.Ststistical significance was found between the two groups using Log-Rank test(P＝0.014). Univariate regression models and multivariate regression models were used to analyse the possible factors influence prognosis including age,tissue types,clinical stages,histological differentiation and ascites.The result was no factors but clinical stages to be an independent adverse prognostic factor.4.The expression of Sema4C mRNA in human ovarian cancer cell was higher than that in human fibroblast cell.And the expression of Sema4C protein in human ovarian cancer cell was positive while that was negative in human fibroblast cell. Conclusion1.The expression of Sema4C in the group of epithelial ovarian cancer was significant higher than that in benign,normal and borderline group.The over expression of Sema4C is correlated with the origination,progression and the biological behavior in human epithelial ovarian cancer.The expression level of the Sema4C can be used as an auxiliary diagnosis of human epithelial ovarian cancer.Sema4C may become a new target for gene treating of ovarian cancer.2.The overexpression of Sema4C was negatively related with survival rate of ovarian cancer patients.3.The expression of Sema4C mRNA in the ovarian cancer cell was higher than that in normal cell.And the expression of Sema4C protein in the ovarian cancer cell was positive.This will help us to find out the relationship between Sema4C and biological behavior of ovarian cancer.