Correlations Of AT₁R Gene Polymorphism To The AT₁R MRNA And Protein Levels In Brain Tissue After Hypertensive Cerebral Hemorrhae

ObjectiveTo investigate the association of angiotensin Ⅱ type Ⅰreceptor （AT₁R）gene polymorphism with AT₁R expression levels and brain edema inhypertensive intracerebral hemorrhage.Methods45patients of hypertensive intracerebral hemorrhage undergoingoperations were collected from December,2011to August,2012in theNeurosurgery of Yongchuan Hospital, Chongqing Medical University:1.The AT₁R A1166C polymorphism was assayed by restrictionfragment length polymorphism （RFLP） analysis;2.Brain tissue was taken from lesions in operation, then AT₁R mRNAconcentration was determined by RT-PCR and AT₁R protein level wasdetermined by Western blot; 3.Cerebral edema was evaluated by a non-invasive brain edemamonitor at24h,48h and72h after operations.Results1.There were36cases of AA genotype,9cases of AC genotype, andthe frequency of A and C alleles was90%and10%respectively;2.The concentration of AC genotype AT₁RmRNA were slightly higherthan the AA genotype（P=0.52）, but the difference was not statisticallysignificant;3. The concentration of AC genotypes AT₁R protein in brain tissuewas significantly higher than the AA genotype （P=0.011）;4. No significant differences were found in the cerebral edema factorsbetween the two genotypes within24hours （P=0.226）, but significantdifferences were found in48hours （P=0.034） and72hours （P=0.005）.Cerebral edema factor in the AC genotype is higher than the AA genotype.Conclusion：1.There was no significant correlation between the AT₁R genepolymorphism and AT₁RmRNA concentration in the brain tissue;2.There were significant correlations between AT₁R genepolymorphism and AT₁R protein concentration in the brain tissue, AT₁Rprotein concentration of the C allele carriers was higher;3.There were significant correlations between AT₁R genepolymorphism and the extent of cerebral edema in hypertensive cerebral hemorrhage, the more frequent occurrence of cerebral edema were foundin the C allele carriers.