| ObjectiveTo analyze the tendency and antimicrobial resistance of clinical isolatesin our hospital from2008to2010, which will provide the basis for rationaluse of antimicrobial drug for clinicai.MethodsDisk diffusion method was used to determine the antimicrobialsusceptibility. And the WHONET5.4software was used to analyze the databased on the annual CLSI.ResultsOf4916clinical isolates, gram-positive organisms accounted for22.7%, and gram-negative organisms77.3%. The detection rate ofmethicillin-resistant Staphylococcus aureus (MRSA) was59.3%, the yearlyrates were56.8%,63.7%,56.7%, respectively; and the detection rate ofmethicillin-resistant coagulase-negative staphylococci (MRCNS) was89.5%, the yearly rates were81.7%,93.8%,91.9%, respectively. Thesensitivity of MRSA and MRCNS was100%to teicoplanin, vancomycin,linezolid. The yearly ratios of Enterococcus faecail and Enterococcus faecium were97.7%,92.5%,120%, respectively. Two strains ofEnterococcus faecium were found resistant to vancomycin and teicoplanin.Total Extended-spectrum β-lactamase（sESBLs）-producing Escherichia coliwas accounted for57.5%, each year was51.1%,57.2%,61.7%; totalExtended-spectrum β-lactamases（ESBLs）-producing Klebsiella spp wasaccounted for34.6%, each year was27.8%,35.4%,38.8%. The resistancerates of Enterbacteriaceae bacteria to imipenem and meropenem werebelow5%. Each year the resistance rate of acinetobacter Spp to imipenemwas36.5%,55.4%,68%, respectively; the rate to cefoperazone/sulbactamwas3.7%,17.3%,57.5%, respectively. For three years the resistance ratesof Pseudomonas aeruginosa to amikacin were39.3%,18.1%,17.7%; andthe rates to cefoperazone/sulbactam were10.9%,10.7%,25.0%,respectively.ConclusionThe gram-positive bacteria were still susceptible to vancomycin,teicoplanin and linezolid in our hospital, but Two strains of Enterococcusfaecium were found resistant to vancomycin and teicoplanin in2010.Enterbacteriaceae bacteria are still very sensitive to carbapenems.However, the ESBLs-producing strains in Enterobacteriaceae aremore resistant than none ESBLs-producing strains. The carbapenemresistance rate in Acinetobacter Spp has increased year by year,especially to carbapenems and cefoperazone/sulbactam. For Pseudomonas aeruginosa, amikacin and cefoperazone/sulbactam are the most potentantimicrobial. |
