| Objective Over the past decade, the rapid growth in the number ofpatients with type2diabetes mellitus with serious hazard to human’s health.One of gastrointestinal surgery-gastric bypass that can improve diabetesand it has become the focus of people’s attention. In view of the importantrole about the Fox01factor that stand in the pathogenesis of diabetes andobesity. We study the alteration on expression of Fox01on the liver andpancreas in type2diabetic rats which undergone gastric bypass surgery. Weaim to explore the mechanism for gastric bypass on type2diabetesMethods A number of12-week-old Sprague-Dawley rats fed highfat mash for four weeks then rats were intraperitoneal injected30mg/kgstreptozotocin (STZ).30successful diabetes model rats were randomlydivided into gastric bypass group (group A,n=10),sham operationgroup(group B,n=10) and diabetes group(group D,n=10). Those rats didn’tinjected STZ were divided into normal control group(group C,n=10). The levels of fasting serum insulin and glucagon-like peptide-1(GLP-1) weremeasured and compared among the A and B groups before the operation andat4,8,16,24weeks following the operation, those items were also measuredin C and D group. After6months, all rats were decapitated to retrievelivers,pancreas and surveyed the expression of the Fox01by usingimmunohistochemistry and western-blotting.Result Group A rats postoperative blood glucose show a gradualdownward trend:4weeks after gastric bypass surgery the fasting bloodglucose changed from (20.2±2.1) mmol/L to (10.5±2.5) mmol/L;8weeks after surgery:(10.2±2.1) mmol/L;16weeks after surgery:(8.2±1.3)mmol/L;24weeks after surgery the fasting glucose reduced to (7.7±1.1)mmol/L, but the average value is still higher than that in group C (p <0.05).Fasting glucose on group B rats four weeks after the sham operation was(19.2±2.0) mmol/L but glucose of the preoperative was (18.3±6.1) mmol/L,8weeks after surgery:(22.5±3.2) mmol/L, surgery after16weeks:(21.9±2.7) mmol/L, and after24weeks it rose to (22.9±2.8)mmol/L.Group C rat was used the high fat diet for1month, didn’t do the surgery.Fasting blood glucose at the different time points respectively were:(3.6±0.4) mmol/L;(3.6±0.3) mmol/L;(3.6±0.3) mmol/L;(3.4±0.3) mmol/L;(3.5±0.5) mmol/L. Blood glucose throughout the course of our studyhas been in normal range.Group D rats were the diabetes but did not undergoany treatment such as surgery, the phenomenon that their blood glucose increased slowly over time, fasting blood glucose at the different time pointswere:(20.0±2.1) mmol/L;(21.4±2.6) mmol/L;(22.4±3.0) mmol/L;(24.1±3.7) mmol/L;(25.6±3.4) mmol/L.Group A rats’s fasting seruminsulin (Insulin) about preoperative,postoperative on4weeks,8weeks,16weeks and24weeks, respectively, were (2.87±0.08) mU/L,(2.91±0.08)mU/L,(2.88±0.08) mU/L,(2.95±0.08) mU/L and (3.00±0.06) mU/L. Itkeeped slowly increasing trend. The measurements about fasting serumGLP-1on corresponding time point, respectively, were (8.7±0.9)pmol/L,(13.2±1.0) pmol/L,(16.1±0.2) pmol/L,(18.4±0.7) pmol/L and(23.5±0.2) pmol/L. Compared with the same period of B and D group, theGLP-1of Group A rats after surgery was significantly higher(p<0.05).Insulin measurement results of C group at the corresponding timepiont, respectively, were (3.71±0.07) mU/L,(3.68±0.11) mU/L,(3.70±0.08) mU/L,(3.63±0.11) mU/L and (3.68±0.14) mU/L; and the results ofGLP-1were (17.9±0.1) pmol/L,(19.7±0.3) pmol/L,(21.4±1.2) pmol/L,(18.1±0.4)pmol/L and (19.9±1.0) pmol/L.Group D’s serum insulin weremU/L (2.76±0.09),(2.74±0.10) mU/L (2.70±0.10) mU/L (2.69±0.12)mU/L (2.70±0.11)mU/L and the results of GLP-1, respectively were (7.7±0.5)pmol/L,(6.1±1.0)pmol/L,(4.4±0.2) pmol/L,(5.2±0.8) pmol/L and(4.9±0.7)pmol/L.Rats in group A and B after gastric bypass24weeks therelative amount on expression of Fox01in liver, respectively,were0.92±0.02B rats,1.865±0.18.This value on group C and D were0.46±0.01,1.79 ±0.15. Fox01’s expression of group A rat in liver was significantly lowerthan B and D group’s and the discrepancy was statistically significant (p<0.05)Expression of Fox01protein in pancrea’s islet about each groups ofrats was not exactly same. Rat of group A that their Fox01’s expression inpancreatic mainly manifested in the cytoplasm. However, the expressionFox01main displayed strongly positive in the nucleus of β-cell in rats’pancreatic of group B, while the positive-expression showed in cytoplasm.The similar changes happened in group B and D. The expression of Fox01in pancreas of rats of C group was weakly positive, it mainly distributed inthe cytoplasm.Conclusion Type2diabetic rats as an animal model that inducedby high-fat diet combined STZ injection could used to be a wonderful toolfor the study of gastric bypass in the treatment of diabetes. Gastric bypasssurgery shows a good effect in the treatment of type2diabetic rats inducedartificially, to some extent, it manifested a hypoglycemic effect. Theelevated serum GLP-1speculated postoperative and alterations about theexpression of Fox01factor in liver and pancreas may be one of themechanisms that gastric bypass surgery for the treatment to type2diabetesmellitus. |
PDF Full Text Request