The Effect Of Intraperitoneal Catheter Drainage To Ca²⁺ Overload In Pancreatic Acinar Cell Of Rats With Severe Acute Pancreatitis

Objective To investigate the effect of intraperitoneal catheterdrainage to Ca²⁺overload in pancreatic acinar cell of rats with severe acutepancreatitis by setting an intraperitoneal catheter drainage to the rats.Methods Seventy-two male Wistar rats were equally randomizedinto the sham operation group, SAP model group and catheter drainagegroup. The last two groups were induced by retrograde cholangiopancreaticduct injection of3%sodium taurocholate, and then catheter drainage groupwas induced by placing an intraperitoneal catheter drainage for drainageof ascites. The three groups of rats were put to death in the postoperative6h,12h,24h sentinel（eight mice at each time point）. Take venous blood andascites to detect interleukin hormone（IL）-8, tumor necrosis factor（TNF）-ɑinflammatory cytokines thromboxane（Tx）A₂and amylase（AMY）,phospholipase（PL）A₂activity, take pancreatic tissue to detect theconcentration of [Ca²⁺]iin acinar cell and make HE staining biopsy.Results At6h,12h,24h three time points，the serum IL-8, TNF-ɑ, AMY, TxA_<sup>2+2, pancreatic tissue PLA₂and acinar cells[Ca]iof catheterdrainage group were significantly lower comparing to the SAP group.Conclusion Drainage of the inflammatory cytokines IL-8, TNF-ɑ,etc, by setting an intraperitoneal catheter drainage to rats, can reducegeneration of TxA₂、LT and activity of PLA₂, reduce the injury to thepancreas, and this is maybe owing to the alleviate of calcium overload inpancreatic acinar cells.