The Influence Of Sijunzitang Demolition Party To The Proliferation And Apoptosis Of Gastric Cancer SGC-7901，BGC-823Cell Line Side Population Cells

Purpose: Using the side population cells of Gastric cancer SGC-7901and BGC-823cell lines which were sorting out by Flow cytometry as the research object, detect theproliferation,apoptosis,cycle,expression of apoptosis-related proteins of the two celllines which were affected by Sijunzitang demolition party.Conbinition the effects ofSijunzitang whole side, clarify the mechanism Sijunzitang acting on the side populationcells,and lay the foundation for the subsequent experiments.Method:1. Routinely cultured human gastric cancer cells in vitro, including heightdifferentiated gastric cancer cell line MGC-803, differentiated gastric cancer cellline SGC-7901and poorly differentiated gastric cancer cell line BGC-823.2. Sort out the side population cells of SGC-7901and BGC-823of gastric cancercells,and detecting the side population cells of MGC-803cell line.3. Preparation the drug serum of Sijunzitang demolition parties.4. Using CCK-8detect the proliferation change of side population cells、non-sidepopulation cells and unsorted cells before or after the intervention of the drugserum of Sijunzitang demolition party.5. Detect the change of cell cycle and cell apoptosis of side population cellsinterposed by drug serum by Flow cytometry.6. Using Western blot detect the expreesion of apoptosis-related proteins Bax andBcl-2of side population cells,which were interposed by drug serum.Result:1. Sorting out the side population cells of MGC-803cells of6samples, the separationratio of which is0%.2. After168hours of the drug serum intervention,the proliferation of side population cells was below which of the saline control group. There is significant differencesbetween the two groups(p<0.05).But much higher than the group of non-sidepopulation cells(p<0.05, individual group p＞0.05).At the same time, the inhibitionrate of proliferation of side population cells was lower than the group of non-sidepopulation cells,the differences is obvious(p<0.05).3. With extension of time,especially point to drug serum impact on cells,theproliferation rate of side population cells rised gradually(p<0.05),but generallylower than the group of non-side population cells(p<0.05,individual group p＞0.05).4. As the extension of time that drug serum impacted on cells,the rate of G1stage ofside population cells rised gradually, the rate of G2and S stage reducedgradually.After the intervetion of drug serum, the rate of G1stage of sidepopulation cells was lower than the the group of non-side population cells, the rateof G2and S stage was higher than the the group of non-side population cells. Theabove comparison difference is more apparent,(p<0.05,individual group p＞0.05).5. With elevated drug serum concentrations and prolonged duration of action,theexpression of Bax of the side population cells rised gradually than the Salinegroup,but generally lower than the group of non-side population cells(p<0.05,Individual group p＞0.05). The expression of Bcl-2of the side population cellsreduced gradually than the Saline group, but generally higher than the group ofnon-side population cells(p<0.05, individual group p＞0.05).Conclusion：1. Drug serum of Sijunzitang demolition can inhibit the SP gastric cancer cell’Sproliferation, the mechanism of which was the change of cell cycle.2. Drug serum of Sijunzitang demolition can promote the apoptosis of SP cellls, thepossible mechanism of which was the Upregulation of pro-apoptotic protein-Baxand downof Anti-apoptotic protein-Bcl-2.