Hypolipidemic Effects Of Phloridzin In Hyperlipidemia Hamsters

Phloridzin is a dihydro-chalcone glycoside in plant flavonoids, with a variety of biological activity. Studies have shown that phloridzin may prevent and treat diabetes, have antioxidant ability and improve memory. In this paper, hamsters as the research model,hypolipidemic effects, antioxidant of hyperlipidemic hamsters and hypolipidemic mechanism of phloridzin was mainly studied.In this paper, we determined purity of the sample phloridzin by high-performance liquid chromatography method. The column was C18column. A binary H2O-0.1%H3PO4(solvent A) and acetonitrile(solvent B) gradients was used for elution.Detection wavelength was280nm. It was showed that the purity of sample phloridzin was75.02%.By set the model of hyperlipidemia hamsters and fed in hyperlipidemia hamsters different doses of phloridzin, we studied the effect and mechanism of phloridzin hypolipidemic.0.3%-0.9%phloridzin adding dietary may significantly reduce the value of total cholesterol(TC), triglyceride(TG) and non-high density lipoprotein cholesterol(non-HDL-C) in serum, increase high density lipoprotein cholesterol(HDL-C) in serum, and also reduce the overall target TG/HDL-C and atherogenic index(AI) of lipid levels. Dose groups compared with model group, the liver TC and TG levels were significantly reduced. This indicates that phloridzin has hypolipidemic effect.Antioxidant indicators for the determination of hamsters, the results show that phloridzin can significantly reduce the MDA formation of lipid peroxide in serum, liver, heart and kidney of hyperlipidemia hamsters. Dose groups compared with model group, SOD activity, CAT activity, GSH-PX activity and T-AOC in serum, livers, hearts and kidneys of hyperlipidemia hamsters were increased, only T-AOC in serum is not significant, the rest were significant differently. Therefore it shows that phloridzin has good antioxidant resistance.Phloridzin can significantly reduce blood glucose levels of hyperlipidemia hamsters. Dose groups compared with model group, Cholinesterase(CHE) activity was significantly enhanced, it shows that phloridzin can reduce the liver injury to a certain extent.By measuring the enzymes of hamster liver mRA expression related to cholesterol metabolism, such as HMG-CoA reductase, LDL-R and CYP7A1, hypolipidemic mechanism of phloridzin was studied. The results of experiments showed that phloridzin possess hypolipidemic activity and antioxidant activity.