Optimization Of Extraction Process Of Phloridzin From Lithocarpus Polystachyus Rehd And Antioxidant And Hypoglycemic Effects

Lithocarpus polystachyus Rehd,commonly known as Sweet Tea.Phlorizin is acompound extracted from L.polystachyus.Phlordzin has a wide range of pharmacological activities.In this paper,the natural active products in L.polystachyus are further studied to provide more theoretical basis for the efficient utilization of L.polystachyus resources.The study is divided into the following six parts:1.Optimization of ultrasound-assisted extraction of phloridzin from L.polystachyusUltrasonic-assisted extraction method was used to extract phlorizin from L.polystachyus.The RSM was performed and the optimal conditions for extraction were obtained:volume fraction of ethanol 64%,liquid-solid ratio 37:1,soaking time 35 h and sonication time 38 min.The proportion of the extraction of phloridzin from L.polystachyus under this industrial process conditions was 3.83%.2.Isolation and purification of antioxidant PhlorizinThe optimum conditions for the purified phloridzin of macroporous resin were investigated by static and dynamic tests.And the antioxidant activity of phloridzin was determined by three methods.The optimal parameters for purification were as follows:The the sample concentration of 2 mg/mL,sample volume of 180 mL,the sample flow rate of 2BV/h,ethanol concentration of 75%,eluting agent volume of 4 BV,desorption rate of 2BV/h.By this technology the phloridzin content was increased from 27.62%of the crude extract to 72.37%of the purified product.The IC₅₀ of hydroxyl radical was 345?g/mL.It is suggested that phloridzin have good antioxidant activity.3.Established hyperglycemia model of SD ratsSD rats were induced by intraperitoneal injection of high-fat diet and different doses ofSTZ to establish an animal model of T2DM.After modeling,the stability of the model was observed for 28 days.The results showed that STZ of 40 mg/kg intraperitoneal injection was the optimal dose to establish rat hyperglycemia model.4.Preventive effect of phlorizin on diabetic ratsSD rats were divided into six groups.The STZ model was injected into the abdominal cavity after continuous gavage.To observe the prophylactic effect of phloridzin on hyperglycemic rats.The results showed that phloridzin can reduce the blood lipid blood glucose level in rats,reduce the metabolic indexes,and protect the liver and pancreas of rats,suggesting that oral root picoside can prevent and delay the hyperglycemia caused by STZ.5.Phlorizin treatment of diabetic ratsThe rats were randomly divided into six groups.After successful modeling,rats in each group were given 28 days of continuous gavage,to observe the therapeutic effect of phloridzin on hyperglycemia rats.The results showed that phloridzin can reduce blood lipids and blood glucose levels in hyperglycemic rats,and improve the clinical symptoms,and protect the liver and pancreas.This shows that phloridzin has a hypoglycemic effect.