Experimental Study Of Extract From Rhizoma Phragmitis To Prevent Calcium Oxalatestones In Male Rats

ObjectiveObserve the influence of Extract from Rhizoma Phragmitis in male ratsrenal calcium Oxalate.The purpose of this paper was to obtain purpose of thispaper was to obtain.MethodsForty Health male SD rats were randomly divided into five groups（N=8each）,they were control, model, and Rhizoma Phragmitis interventiongroups.After experiment4weeks, the24h urine concentration Ox, Ca²⁺, theconcentration of serum BUN, Cr, Ca²⁺, P and MDA, SOD of renal tissue weredetected in each of group rats, The calcium oxalate deposit in the kidney wasobserved by microscopy. Immunohistochemistry were used to assess theprotein expression of OPN in rat renal tissue of every groups.ResultsThe24h urine concentration Ox, Ca²⁺and serum Cr of Rhizoma Phragmitisintervention group was lower than stone formation group obviously（P<0.05）.Rhizoma Phragmitis intervention group the enzyme activity of superoxidedismutase（SOD）of renal tissue was higher than stone formation group（P<0.05）;Stone formation group concentration of malondialdehyde（MDA） of renal tissuewas higher than Rhizoma Phragmitis intervention group（P<0.05）. In rats ofnormal control group, only a weak OPN protein staining was noted. Theexpression was enhanced in the stone group. Extract from Rhizoma Phragmitis-treated rats tended to have lower OPN protein expression than the stone group. ConclusionsThe extracts from Rhizoma Phragmitis can block the formation of renalcalcium oxalate stone in rats potently.