TITLE The Relationship Between Memory T Cells And Clinicopathological Factors And Prognosis Of Gastric Cancer

Objective:To investigate the relationship between the distribution and quantity of CD45RO+T cell, CD11c+dendritic cells(CD11c+DCs) and CD8+T cell in the tumor microenvironment and the prognosis of gastric cancer. To explore the changes of memory T cell(MTC) and DCs subsets during the periopeation, and to investigate the relationship between MTC and clinicopathologic factors.Methods:1From January2000to January2001, the paraffin blocks of60cases of gastric cancer were collected from Tianjin cancer hospital. Immunohistochemistry was used to detect the expression of CD45RO+T cell, CD11c+DCs and CD8+T cell in previous tissues. We investigated the relationship between them and prognosis.2From June2009to December2009, the MTC subsets and DCs subsets of gastric cancer patients during the periopeation were measured by Flow cytometry. To explore the changes of MTC and DCs subsets of gastric cancer patients during the periopeation. And to investigate the relationship between MTC and clinicopathologic factors. And chosed30cases of healthy volunteers as healthy controls.Results:1The expression of CD45RO+T cell was closely associated with lymph nodes metastasis (P<0.01) and clinical stage(P<0.05). And the dengsity of CD11c+DCs and CD8+T cell in the tumor microenvironment were closely correlated with clinical stage(P<0.05and P<0.05).2Kaplan-Meier survival analysis revealed that patients in the high-density groups for CD45RO+T cell (P<0.01), CD11c+DCs (P<0.05) and CD8+T cell (P<0.05) had a significantly longer survival time than that in the corresponding low-density groups, respectively. In multivariate survival analysis, the densities of CD45RO+T cell and lymph metastasis remained independent prognostic factors.3Compared to healthy controls, there was an decrease in myeloid dendritic cells(mDCs) and plasmacytoid dendritic cells (pDCs) in preoperative gastric cancer patients (P<0.05and P<0.01). Preoperative gastric cancer patients had statistically higher percentage of CD4+MTC and CD8+MTC in peripheral blood than healthy controls(P<0.01and P<0.01); had statistically lower percentage of CD4+central memory T cell (TCM) and CD8+central memory T (TCM) cell in peripheral blood than healthy controls (P<0.01and P<0.01); and had statistically higher percentage of CD4+effector memory T cell (TEM) and CD8+effector memory T cell (TEM) in peripheral blood than healthy controls (P<0.01and P<0.01).4After surgical removal of tumor, there was an increase in mDCs in peripheral blood gastric cancer patients (P<0.05), but no significance was found in pDCs and all other MTC subsets5The significant correlations were observed between mDCs and CD4+MTC (rs=0.548,P<0.01).6The percentage of CD8+MTC in peripheral blood was closely associated with lymph nodes metastasis (P<0.01) and clinical stage(.P<0.01), and the percentage of CD8+TCM in peripheral blood was closely associated with clinical stage(P<0.05).Conclusion:1Density of immunocyte was found to be predictive of lymph node metastasis. And the densities of CD45RO+T cell can be used as an independent prognostic factor.2There was an decrease in mDCs and pDCs in preoperative gastric cancer, and there was an increase in mDCs after surgery, which suggested that cellular immunity may be related to tumor burden.3Preoperative gastric cancer patients had statistically higher percentage of CD4+MTC and CD8+MTC in peripheral blood than healthy controls, suggesting that immune memory against gatric cancer have formed in vivo.4mDCs correlates positively with CD4+MTC significantly, suggesting that mDCs may contribute to the development of CD4+MTC.5The percentage of CD8+MTC in peripheral blood was closely associated with lymph nodes metastasis and clinical stage, suggesting that CD8+MTC contribute to metastasis and progression of gastric carcinoma.