Experimental Study On Expressions Of MTA1,BRMS1,GRIM19and GW112in Ovarian Endometriosis Cyst Tissue

Objective To investigate the relationgship between metastasis associated gene1(MTA1), breast metastasis suppressor1(BRMS1), genes associated with Retinoid-IFN-induced Mortality (GRIM19), olfactomedin4(GW112) and the biological behaviors of endometriosis. Methods Forty inpatients with endometriosis accepted laparoscopic surgery and the specimen collect at department of Obstetrics and Gynecology Affiliated Haikou Hospital of Xiangya Medical School of Central South University from July in2011to July in2012, whose in eutopic and ectopic tissuese were took in operation at the same time. There were20cases in proliferate phase,20cases in secretory phase,15cases in mild group (I-II),25cases in severity group (III-IV). The normal endomertium were investigated as the normal endomertium group. All the patients were collected at radom. Normal control group: Inpatients with Uterine fibroids accepted laparoscopic surgery during the same period. We used immunohistochemical method to examine the expression of MTA1, BRMS1, GRIM19and GW112protein then analyed the results combined with the clinic pathologic features. Results1, metastasis gene MTA1:①The expression of MTA1protein was found at three groups of different endometrial tissue.②The expression rate of MTA1protein was increased gradually in normal tissue, in ectopic endometrium and ectopic endometrial tissue which was5%,52.5%,80%respectively and there was significantly difference between every two groups, P<0.05.③There were no statistically significant difference between MTA1expression levels in the proliferative phase and the counterparts in secretive phase, P>0.05.④The expression rate of severe group (Ⅲ-Ⅳ period) is higher than mild group (Ⅰ-Ⅱ period) both in ectopic or ectopic endometrial tissues. The difference have statistical significance, P<0.05.2,Metastasis suppressor gene BRMS1:①BRMS1express in endometrial glandular epithelium cells and (or) interstitial cells in the cytoplasm mainly.②The expression rate of BRMS1protein was decreased gradually in normal tissue, in ectopic endometrium and ectopic endometrial tissue which was75%,32.5%,7.5%respectively and there was significantly difference between every two groups, P<0.05.③There were no statistically significant difference between BRMS1expression levels in the proliferative phase and the counterparts in secretive phase, P>0.05.④The expression rate of severe group (Ⅲ-Ⅳperiod) is lower than mild group (Ⅰ-Ⅱ period) both in ectopic or ectopic endometrial tissues. The difference have statistical significance, P<0.05.3, apoptosis gene GRIM19:①The expression of GRIM19protein was found at three groups of different endometrial tissue.②The expression rate of GRIM19protein was increased gradually in normal tissue, in ectopic endometrium and ectopic endometrial tissue which was85%,47.5%,20%respectively and there was significantly difference between every two groups, P<0.05.③There were no statistically significant difference between GRIM19expressing levels in the proliferative phase and the counterparts in secretive phase, P>0.05.④The expression rate of severe group (Ⅲ-Ⅳ period) is lower than mild group (Ⅰ-Ⅱ period) both in ectopic or ectopic endometrial tissues. The difference have statistical significance, P<0.05.4, apoptosis suppressor gene GW112:①GW112express in endometrial glandular epithelium cells and (or) interstitial cells in the cytoplasm mainly.②The expression rate of GW112protein was decreased gradually in normal tissue, in ectopic endometrium and ectopic endometrial tissue which was32.5%,67.5%,92.5%respectively and there was significantly difference between every two groups, P<0.05.③There were no statistically significant difference between GW112expressing levels in the proliferative phase and the counterparts in secretive phase, P>0.05.④The expression rate of severe group (Ⅲ-Ⅳ period) is higher than mild group (Ⅰ-Ⅱ period) both in ectopic or ectopic endometrial tissues. The difference have statistical significance, P<0.05. Conclusion:①The expression rate of MTA1protein was increased gradually in normal tissue, in ectopic endometrium and ectopic endometrial tissue. The expression rate of BRMS1protein was decreased gradually. It is related r-AFS closely but Menstrual cycle. So MTA1/BRMS1is likely to be a couple of negative control protein which play a certain role in EMs endometrial cells on the process of transfer.②The expression rate of GRIM19protein was decreased gradually. The expression rate of GW112protein was increased gradually in normal tissue, in ectopic endometrium and ectopic endometrial tissue. It is related r-AFS closely. So GRIM19/GW112is likely to be a negative control protein which play a certain role in EMs endometrial cells on the endometrial cell proliferation and apoptosis.