| Objective:to study the effection of Anticholinergic drugs on Thl/Th2cells and Treg cells in the mouse model of allergic rhinitis and further explore the possible causes of Thl/Th2imbalance after estab-lishing the mouse model of allergic rhinitis.Methods:Twenty-four,6-8weeks old, Balb/c mice were randomly divided into four groups:control group, model group, model treatment group and control treatment group. After the establishment of mouse model of allergic rhinitis, mice in the treatment group and the control treatment group received intranasal ipra-tropium bromide therapy for fourteen days, and the other two groups were drop intranasally by saline solution. After the last nasal drop, be-havioral scores were evaluated by symptoms of mice, and then killed the mice to collected the serum and nasal mucosa of mice.ELISA was used to measure the concentration of OVAsIgE in serum; Meanwhile HE staining was used to observe the morphological changes of the nasal mucosa,and immunohistochemical technique was adopted to measure the changes in number of the IL-4, INF-γ, FoxP3, VIP and SP in nasal mucosa.Results:l.The behavioral scores and the concentration of OVA-sIgE in mice’s blood serum of the model treatment group were signifi-cantly lower than that one of the model group (p<0.01), and higher than that one of the control treatment group and the control group (p<0.01). In contrast,there are no significant differences between the control treatment group and the control group.2.The positive cells percentage of the IFN-y and Foxp3in nasal mucosa in model group were significantly higher than that of model group(p<0.01),and was significantly lower than that of the control treat ment group and the control group (p<0.01);there were no significant differences between the control treatment group and the con-trol group (p>0.01).3.The positive cells percentage of the IL-4、VIP and SP in na-sal mucosa in model group were significantly lower than that of model group (p<0.01),and was significantly higher than that of the control treatment group and the control group (p<0.01);there were no significant differences between the control treatment group and the con-trol group (p>0.01).Conclusions:1.Anticholinergic drugs can not only effectively alleviate the runny nose of the mouse model of allergic rhinitis, but also relieved the sneezing、nasal itching and stuffy nose to some extent.2. Anticholinergic drugs can reverse the advantage of the cyto-kines secreted by Th2cells over that by Th1cells,so as to keep them in a balance.3. Anticholinergic drugs can increase the amount of the Treg cells in nasal mucosa of the mouse model of allergic rhinitis, which may be the reason to reverse the advantage of the cytokines secreted by Th2cells over that by Th1cells.13figures,7tables and49references... |
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