| Asthma is a chronic recurrent respiratory disease which is characterized by allergic airway inflammation and airway hyperresponsivess. Its major clinical treatment is the combination therapy of bronchiectasis agents and anti-inflammatory agents. These drugs have an effient influence in treating and alleviating the airway hyperresponsiveness. However, we still can not effectively solve some problems, such as repeated episodes, airway remodeling, or the process of decline in lung function et al. Modern medical practice has found that many traditional Chinese medicine have anti-inflammatory, antibacterial and immune stimulating roles in the body. Clerodendrum bungei, verbenaceae, is the commonly used ethnic herbs which has the effect of promoting blood circulation, heat and dampness, swelling and pain, detoxification et al. Recent studies have found that the extracts of Clerodendrum bungei may have inhibitory effects on confronting delayed-type hypersensitivity and cellular immunity. Our previous research found that Clerodendrum bungei was extracted as three active ingredients, including petroleum ether phase, chloroform phase and the water phase fractions. Activity evaluation results showed that the chloroform fraction can mostly reduce airway and alveolar pathological changes, the inflammatory response and airway hyperresponsiveness induced by ozone. The chloroform fraction was divided into A, B, C, D, E and F six active ingredients by silica gel column chromatography. After the activity evaluation, the activity ingredients B and C could reduce the above pathological changes. The compound1extracted from B and the compound2from C were the same compound. In this study, we extracted and separated the effective monomer of Clerodendrum bungei in curing disease of the airway hyperresponsiveness, identified the compound structure of the monomer, and further studied its pharmacological mechanism to provide scientific and reliable basis for clinical medication.Object:To extract and separate the effective monomer of Clerodendrum bungei, identify its chemical structure and explore the pharmacological mechanism at the cellular level.Methods:①The Clerodendrum bungei was successively extracted by petroleum ether and chloroform, separated by silica gel column chromatography and polyamide column chromatography, purified by HPLC to obtain the effective monomer. Using ultraviolet spectrum analysis, infrared spectrum analysis, C spectroscopic and H spectroscopic methods etc to analyze the structure of compound.②Using MTT to detect the effect of the effective monomer of Clerodendrum bungei on the vitality of human bronchial epithelial cells.③HBECs cells were pretreated by the effective monomer of Clerodendrum bungei at different concentrations (0.1-25ug/ml), subsequently, to observe the effect of HBECs on the vitality of ROS, LDH, Catalase,5-LOX under the stress of O3and LPS.Results:①The effective monomer of Clerodendrum bungei was quinone compound which was analyzed by ultraviolet spectrum analysis, infrared spectrum analysis, C spectroscopic and H spectroscopic methods etc.②The effective monomer of Clerodendrum bungei has no significant effects on the vitality of HBECs at the concentration from0.1to25ug/mL.③The effective monomer of Clerodendrum bungei could significantly relieve the increased activity of ROS and LDH induced by O3, and simultaneously increase the vitality of Catalase.④The effective monomer of Clerodendrum bungei could significantly decrease the vitality of5-LOX in HBECs induced by LPS.Conclusion:The effective monomer of Clerodendrum bungei for the treatment of airway hyperresponsiveness disease is quinone compound. This effective monomer could improve the ability of anti-oxidation, anti-injury in human bronchial epithelial cells and weaken the inflammation by inhibiting the activity of5-LOX. |
PDF Full Text Request