The Research Of Rana Skin Chemical Analysis And Antioxidant Activity

Object Analysis the main ingredient Rana leather type, the maincomponent of which extraction and analysis and content inspection, toestablish a scientific basis for the development of Rana leather products.Method The main component of the system pre-experiment analysisRana skin; Worssner using colorimetric analysis of collagen content;Elson-Morgan method and analysis of the content of hyaluronic acid carbazolemethod; using GC-MS analysis of Rana skin and fatty acid composition; usingHPLC Rana skin in estradiol levels; DPPH clearance analysis visits Ranaleather four different extraction methods resulting antioxidant activity of theextracts.Result Through the system pre-experiment analysis Rana skincontains amino acids, protein, saponins, carbohydrates, alkaloids, oils, fattyacids, temperament14kinds associated with skin Rana23kinds of fatty acids,unsaturated fatty acids, unsaturated fatty acids content of85.46﹪of the totalfatty acid content of polyunsaturated fatty acids, five kinds ofmulti-ethylenically unsaturated fatty acid and saturated fatty acid content ratio,i.e. P/S value of1.28； the Rana percutaneous10﹪Na2CO3soaked for30min，water bath digestion8H， collagen protein content378.6mg/g measured in thiscondition Rana leather quality percentage less than37﹪； using waterextraction and alcohol precipitation extraction the hyaluronic acid crude0.7365g, yield1.47﹪, Rana skin transparent a quality acid content of not lessthan0.3835mg/g； Rana skin in estradiol an content for0.2013μg/g；temperature leaching method to extract, freeze-drying the resulting extractbetter antioxidant activity of water measured Rana leather extract on DPPHclearance rate was up to25.6﹪.Conclusion Through this research can confirm Rana leather is a nutrient rich, biologically active products of high economic value, its aqueousextract on DPPH clearance rate was25.6﹪, Rana peel contains amino acids,proteins, saponins, sugar, alkaloids, oils, fatty acids, these ingredients havevery good value in use, suggesting that Rana skin a variety of substances canbe used as raw materials. Collagen is a cosmetic, pharmaceutical, food is oneof the products, Rana skin collagen protein mass fraction of37﹪can be usedas a good raw material for collagen. Rana skin also contains hyaluronic acid,collagen and hyaluronic acid in combination with anti-aging, improve thepassage of time bring women dry skin, wrinkles, loose skin and othersymptoms, suggesting Rana leather is a good beauty products. Rana leather inrich unsaturated fatty acids,14kinds of unsaturated fatty acids, includinglinoleic acid, AA, DHA, EPA, these substances have an important positive rolein the growth and development of the human body. Rana skin in estradiollevels0.2013μg/g, not as good as the frog oil content, reducing concerns ofexcess estrogen administration.