| ObjectiveThe patient of acute PQ poisoning have a high mortality and their therapy israrely and uncertainty. This study is to investigate the relationship between autophagyand apoptosis in the lung during the acute PQ poisoning in the treatment of UTI andSalubrinal.MethodsAll of the350Wistar rats were provided by the Experimental Animal Center ofJilin University. The rats weight is250+10g, included half male and half female. Therats were randomly divided into seven groups. Control group was randomly dividedwith half male and half female, and treated with1ml saline to intragastricadministration. The rats of acute PQ poisoning was treated with g PQ solution40mg/kg once to intragastric administration, and randomly divided into6group, withhalf male and half female, there are50rats each group. The plan of treatment: TheCon/PQ group of rats was intraperitoneal injected1ml saline twice a day. The PUgroup of rats was intraperitoneal injected UTI (12000IU/kg) twice a day. The Sal5group of rats was intraperitoneal injected Sal (0.5mg/kg) in1st,3rd,5th day after PQpoisoning. The Sal10group of rats was intraperitoneal injected Sal (1.0mg/kg) in1st,3rd,5th day after PQ poisoning. The UTI+Sal5(PUS5) group of rats wasintraperitoneal injected UTI (12000IU/kg) twice a day and Sal (0.5mg/kg) in1st,3rd,5th day after PQ poisoning. The UTI+Sal10(PUS10) group of rats was intraperitonealinjected UTI (12000IU/kg) twice a day and Sal (1.0mg/kg) in1st,3rd,5th day afterPQ poisoning. The lung tissue was obtained on the7th day after poisoning, wasobserved by HE stained. We detected the expression of LC3, Bcl-2， Bax and caspase3by Western Blot and Immunohistochemistry in the rats lung.Result1. The lung tissue of rats has a normal morphological in the Con group. It wasobserved from the group of PQ that the abnormal cellular structure, enlargement in thepulmonary alveoli, leaking a lot of inflammatory cells, increasing thickness in thealveoli wall and bleeding in the local area of lung tissue. There were some differentdestruction in other groups.2. When compared with PQ group, the expression of LC3A/Bobviouslyincreased in the group of PU（P<0.01), decreased in the group of PS5and PS10（P<0.05), and nothing changed in the group of Pus5and Pus10. When comparedwith PQ group, the expression of Bcl-2/Bax increased in the group of PU, PS5, PS10,Pus5and Pus10（P<0.05). When compared with PU group, the expression of LC3A/Band Bcl-2/Bax decreased in the group of PS5and PS10（P<0.05).When comparedwith PS5group, the expression of LC3A/Band Bcl-2/Bax increased in the group ofPus5（P<0.05). When compared with PS10group, the expression of LC3A/BandBcl-2/Bax increased in the group of Pus10（P<0.05).3. When compared with Con group, the expression of Cleaved-Caspase3increased in the group of PQ（P<0.05). When compared with PQ group, theexpression of Cleaved-Caspase3obviously decreased in the group of PU（P<0.01),and decreased in the group of Sal5, Sal10, Pus5and Pus10（P<0.05).4. Immunohistochemistry result: When compared with Con group, the expressionof LC3and Bcl-2obviously decreased in the group of PQ（P<0.05). When comparedwith PQ group, the expression of LC3and Bcl-2has different changes in the group ofSal5, Sal10, Pus5and Pus10.ConclusionThe endoplasmic reticulum stress-autophagy is activated in the cellular of acutePQ poisoning rats lung. UTI can ehanced the autophagy and increased the expressionof Bcl-2and decreased the expression of Caspase3to protect the acute PQ poisoningrats lung. But Salubrinal can decreased the autophagy and increased decreased the expression of Bcl-2, but the mechanism of Salubrinal is unclearly in the acute PQpoisoning rats lung. |
