| BackgroundParaquat (PQ), the most common commodity is called kewuzong (gramoxone). A quick touch off ofherbicides, for people, animals have a strong toxic effect. At present there is no effective antidote toparaquat poisoning, studies on its mechanism of action is not clear, the mortality rate is high. The studyfound that the mitogen-activated protein kinase (MAPK) pathway is an intracellular signaling transductionpathway plays a key role in many thorns cell proliferation, apoptosis, differentiation, transformation andother biological response pathways of p38MAPK is the MAPK leading member of the. Transcriptionfactors (NF-κB) in the expression and regulation of inflammatory mediators in a variety of cytokines, thekey transcription factor involved in body tissue damage and stress, cell differentiation and apoptosis, theprocess of information transfer. TNF-alpha is a single nuclear factor, generated mainly by monocytes andmacrophages, LPS is a strong stimulant. PQ poisoning process of p38MAPK, NF-κB, TNF-alpha in thelung, kidney, liver and other organ damage plays a vital role. The liver is the body’s largest detoxificationorgan, by observing the wire in the livers of paraquat poisoning in rat liver tissue damage and detection ofparaquat poisoning group and Wu ulinastatin treatment group of mitogen-activated protein kinasep38MAPK in content and ash value, transcription factors of NF-κB, tumor necrosis factor, TNF-α contentchange to provide a theoretical basis and experimental basis for clinical.ObjectiveThe main purpose is to investigate the basis of paraquat poisoning in rat liver injury anddrug protection role.MethodsHealthy rats by gavage method, preparation of paraquat poisoning model, experimentalselection provided by the Experimental Animal Center of Jilin University60Wistar rats,weighing (200+20) g, half male and half female. Were randomly divided into three groups,A group of normal control group1ml normal saline and daily saline intraperitoneal injection.Group B paraquat exposure group, the PQ solution50mg/kg orally, the same amount ofsaline, once daily intraperitoneal injection. Group C ulinastatin treatment group, in healthyrats after paraquat poisoning and a half hours I UTI D drug120000iu/kg, twice a dayintraperitoneal injection. Three days after the poisoning of healthy rats,7days,14days,28days for abdominal anesthesia, while the extraction of the liver HE staining andimmunohistochemical staining methods to observe the microstructure of the liver and determination of liver tissue mitogen-activated protein kinase p38(p38MAPK) the contentof transcription factor (NF-κB) in tumor necrosis factor alpha (TNF-alpha) content, andanalyzed statistically to evaluate its significance.ResultsThe experimental results show that early liver damage in rats PQ poisoning is notobvious, poisoning the medium-term liver cell degeneration and necrosis, mitigateapplication ulinastatin liver tissue damage after treatment than before, andimmunohistochemical methods for the determination of the release of p38MAPK contentitsgray value changes of NF-κB and TNF-alpha content changes, found that ulinastatintreatment group than in model group decreased significantly.ConclusionManufacture of paraquat poisoning in rat liver tissue model in this experiment, usingHE staining and immunohistochemical staining methods to understand the content ofp38MAPK in its gray value changes of NF-κB, TNF-alpha content, from the biologicalbehaviorthe role of aspects and cytokines observation of paraquat poisoning, liver damage,and the certificate Mingwu ulinastatin drugs can effectively reduce the degree of paraquatpoisoning provides a theoretical basis for clinical treatment of paraquat poisoning. |
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