| Objective:To explore the mechanism of the lipid-lowering effects of moxibustion by observing the effects of lipoprotein metabolism enzyme activity, hepatocyte low-density lipoprotein receptor expression, after warming moxibustion on chronic hyperlipidemia rats.Methods:SD rats were randomly divided into normal group, model control group, warming moxibustion group, lovastatin group. Normal group rats were fed a normal diet, the other groups were intragastric fat emulsion, modeling after28days. They were warmed moxibustion on "Shenque", double "Zusanli" points for4weeks,10minutes a day.The lovastatin group were gave drug everyday for4weeks, other groups only bundled processing. Automatic biochemical was used to analyze serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c) levels; The method of Nitrate reductase to detect lipoprotein lipase (LPL), hepatic esterase activity (HL); Real-Time PCR method for the determination of low density lipoprotein receptor messenger ribonucleic acid (LDLRmRNA) level in rat liver tissue.Used these indicators to explore the effect of warming moxibustion.Results:â‘ arming moxibustion can significantly reduce hyperlipidemia serum TC, TG, LDL-c content, increase HDL-c content (P<0.01, P=0.648);significantly improve hyperlipidemia rat liver LPLã€HL activity (P<0.05); improve the expression of the LDL receptor gene in the liver(P=0.000).â‘¡Serum TC, TG, LDL-c levels of model control group were significantly higher than normal group and the treatment groups, there were significant differences (P<0.01). HDL-c of model control group was more than normal group, for the reason of body promoting the clearance of cholesterol medication, but no significant difference (P=0.648,P=0.813); Finishing the warming moxibustion treatment, TC, TG, LDL-c were decreased significantly compared with the model group,the difference was significant (P<0.01), HDL-c levels increased (P=0.648).â‘¢In model control group, LPL and HL activity of liver were reduced compared with normal group, difference was statistically significant (P<0.01); Lovastatin treatment can improve the liver LPL and HL activity trends, but no significant difference (P=0.136, P=0.314). Warming moxibustion therapy can significantly improve the liver LPLã€HL activity, and were statistically significant (P<0.01).â‘£LDLR expression in liver cells of model control group rats was suppressed according to normal group and lovastatin group, differences were statistically significant (P<0.01); The warming moxibustion had the able to activate LDLR expression in the liver cells, increase LDLR quantity, promote the liver cells LDLRmRNA transcription, LDLRmRNA expression level was significantly improved (P<0.01). Conclusion:Low-density lipoprotein receptor function defects and liver LPL, HL decreased activity can cause hyperlipidemia. Warming moxibustion can relieve the inhibition state of low density lipoprotein receptor in hyperlipidemia the rats, promote hepatocyte LDLRmRNA expression, increase LDLR activity, promote liver metabolism and clearance of LDL.Also warming moxibustion had the effect of increasing liver LPL, HL active at the same time, speed up the clearance of lipoproteins in the blood, regulation of HDL metabolism, increasing the reverse transport of cholesterol. Warming moxibustion used multi-channel, multi-cellular targets adjust metabolism of lipids, which interfere with the occurrence of hyperlipidemia. |
