Study Of The Relationship Between Oxidized Low-density Lipoprotein And Lectin-like Oxidized Low-density Lipoprotein Receptor-1 And The Pathophysiological Processes Of Preecalmpsia

Objective To investigate the significance of oxidized low-density lipoprotein(oxLDL) and lectin-like oxidized low-density lipoprotein receptor-1(LOX-1) in preecalmpsia.Methods From June 2007 to January 2008,73 women with preeclampsia who delivered in the Department of Obstetrics,Affiliated Hospital of Qingdao University Medical College,were recruited in the study,including 35 women with moderate preeclampsia(MPE group) and 38 women with severe preeclampsia(SPE group).And 45 healthy pregnant women were taken as control group.Enzyme-linked immunosorbent assay(ELISA) was used to measure the plasma concentrations of oxLDL in these women. Semiquantitative RT-PCR and Western blot were used to investigate the expression of LOX-1 mRNA and protein in placenta.The expression of caspase-3 mRNA in placenta was determined using Semiquantitative RT-PCR.Results(1) The plasma concentrations of oxLDL in MPE group(0.42±0.11)mg/L,SPE group(0.68±0.12)mg/L,were significantly higher compared with control group(0.35±0.14)mg/L(P＜0.01) and the concentrations of oxLDL in MPE group were significantly higher compared with SPE group(P＜0.01)(2) The expression of LOX-1 mRNA in placenta of MPE group(0.70±0.10) and SPE group(0.84±0.08) were significantly higher than that in control group(0.58±0.11)(P＜0.01) and the expression of LOX-1 mRNA in MPE group was significantly higher than that of SPE group(P＜0.01)(3) The expression of LOX-1 protein in placenta of MPE group(0.79±0.15)and SPE group(0.90±0.12) were significantly higher compared with control group(0.68±0.11)(P＜0.01),while the expression of LOX-1 protein of MPE group was significantly higher compared with SPE group(P＜0.01)..(4)The expression of caspase-3 mRNA in placenta of MPE group(3.82±0.18) and SPE group(5.39±0.14) were significantly higher than that in control group(2.19±0.20)(P＜0.01 ),and the expression of caspase-3 mRNA in MPE group was significantly higher than that of SPE group(P＜0.01).(5) In preeclampsia groups,the levels of LOX-1 mRNA in placenta were positively correlated with the plasma concentrations of ox-LDL(r=0.93,p＜0.05),and caspase-3 mRNA expression were positively correlated with the expression of LOX-1 mRNA in placenta(r=0.84,P＜0.05).Conclusion increased levels of oxLDL in plasma may induce excess expression of LOX-1 in placenta,which may be involved in the pathoanatomical process of preeclamsia.