Expression Of PTEN And CyclinD1in Human Adenomyosis And The Clinical Significance

SettingAdenomyosis is a kind of benign lesions, which a activity of endometrialglands and stoma invade myometrium and growing in， but it has the nature ofthe malignant tumors. it has become a common gynecological diseases,30-50years old women often see this, the incidence rate is about8.8%-31.0%, and ithas a rising trend in recent years The clinical manifestations are progressivelysecondary dysmenorrhea, menstrual disorders, menorrhagia infertility andanemia, It seriously damages to the patient’s normal life. At present, the diseasetreatment are more, clinical decision need considering into age, symptoms andfertility patients for individualized treatment. In patients with no fertilityrequirements can choose operation treatment, but the lack of effective treatmentmethods for young women with fertility requirements (clinical data prove, drugsfor the treatment of adenomyosis results in general).In recent years, research onuterine glandular myopathy more put forward many kinds of theories about itspathogenesis.But has yet to have a kind of theory to determine which factors inuterine glandular myopathy plays a critical role in development. with the diseasein the exploration, it will deepen their awareness of the disease, and will providecertain theoretical basis for clinical treatment.ObjectiveWe will detect he expression of PTEN, CyclinD1in adenomyosis (AM)patients which includes in ectopic, ectopic endometrium and normalendometrium, by using immunohistochemical method and analysis theexpression differences and relevance of PTEN, CyclinD1.And discuss PTEN,CyclinD1and uterine glandular myopathy occurrence and developmentmechanism of apoptosis, provide certain experiment basis for the exploring and clinical treatment of uterine glandular myopathy.MethodsBetween December2011and July2012, I collect clinical cases at Mounttaishan medical school affiliated hospital and tengzhou center of people’shospital In these cases, I have need to exclude endometrial other lesions,Operation treatment of45cases of patients as experimental group, Adenomyosiseutopic endometrial hyperplasia10cases,13cases of secretory phase; ectopicendometrial hyperplasia15cases,7cases of secretory phase. At the same time,because the operation treatment of uterine fibroids (confirmed by pathologicalexamination after operation for uterine fibroids resection) and the normalendometrium of20cases as control group, including6cases of endometrialhyperplasia,14cases of secretory phase. We will be using immunohistochemicalmethod to determination of PTEN, CyclinD1expression,The data wereprocessed with SPSS13.0software, using Fisher’s exact test and Speannan rankcorrelation analysis was used for statistical analysis of the data, with a=0.05asthe test standard, P <0.05was statistically significant.Results1.The results of immunohistochemistry showed that PTEN, CyclinD1protein in the experimental group (eutopic endometrium, ectopic endometrium)and control group (normal endometrium) positive expression in both.2. The expression of PTEN: PTEN mainly expressed in endometrial glandularepithelial cells of the pulp. The positive expression rate was95.0%(19/20)，65.2%(15/23)，31.8%(7/22)， and showed a trend of gradual decline. PTEN inendometrium positive expression rate in experimental group compared withnormal endometrium expression rate differences statistically significant(P<0.05), In the ectopic endometrium, its negative expression is significantlylower than its in ectopic and normal endometrium, it has statistically significantdifference between the three groups (P<0.05); The expression of PTENhyperplasia in the control group is lower than the production period, thedifference between the two was statistically significant (P<0.05), PTEN inectopic endometrium, eutopic endometrial hyperplasia period of positiveexpression rate is lower than the secretory phase, and there was significantdifference between them (P <0.05). 3. The expression of CyclinD1:CyclinD1staining mainly within the cellnucleus and visible brownish-yellow granules. The positive expression rate were25%（5/20），56.5%（13/23），68.2%（15/22）， a gradually rising trend. CyclinD1in the experimental group in ectopic positive expression rate higher than that ofnormal endometrium, between the two was statistically significant difference(P<0.05), ectopic endometrium positive expression rate was significantly higherin ectopic and normal endometrium, differences between groups werestatistically significant (P<0.05); CyclinD1in the experimental group thoughtlining, stage in ectopic secretion and proliferation expression has no statisticalsignificance (P<0.05), There was no statistical significance of CyclinD1in theeutopic endometrium, ectopic endometrial secretory phase and proliferativephase expression (P>0.05);4． The expression of PTEN negatively correlated CyclinD1(r=-0.585，p=0.000).Conclusion1. PTEN positive expression in ectopic endometrium of adenomyosis of therate is lower than the expression in normal endometrium and eutopicendometrium, low expression of PTEN may be related to the occurrence ofadenomyosis development.2. CyclinD1in the adenomyosis eutopic endometrium, eutopic endometrialtissues with high expression suggest that the high expression of CyclinD1maybe related to carcinogenesis and development of adenomyosis,detected byCyclinD1contributes to the development and prognosis of uterine adenomyosis3. PTEN expression was negatively correlated with CyclinD1, probably dueto the loss of PTEN protein, promote the overexpression of CyclinD1protein,resulting in the occurrence and development of uterine adenomyosis.