| Background and objectiveThe cervical cancer is one of most common gynecologic malignant tumor,its incidence is the first in the developing nation.In recent years the study report demonstrated that the young woman's incidence of cervical adencarcinoma assumed the trend of increase,its proportion has accounted for the cervical cancer case of illness total 19.4%.Because pathogenesis of the cervical adenocarcinoma is unclear, its clinical and the molecular biology characteristic is different with cervical squamous cell carcinoma.In the cervical adenocarcinoma the patient 5 years survival rate is lower,prognosis is worse,therefore cervical adenocarcinoma is focus in gynecology malignant.Oncogenes activation and anti-oncogene inactivation is important in development of tumor.CyclinD1 is a oncogenes to adjust cell cycle.P27 is a important anti-oncogene.At present,several studies had discovered CyclinD1 and P27 abnormal expression in the many kinds of malignant tumor,as gastric cancer, liver cancer,and breast cancer so on.The two kind of protein expression are correlated to the malignant tumor.CyclinD1 and P27 have become a focus.In recent years the research to oncogenes and anti-oncogene concentrates to cervical squamous cell carcinoma.There are very few studies of the molecular level to the cervical adenocarcinoma.It has been not yet seen that the CyclinD1 expression research report in domesticthe and foreign about cervical adenocarcinoma and cervical glandular intraepithelial neoplasia,P27 expression report is very few in the cervical adenocarcinoma and cervical glandular intraepithelial neoplasia.To study expression of CyclinD1,P27 in cervical adenocarcinoma and cervical glandular intraepithelial neoplasia.To analyze the relationships between CyclinD1,P27 and development, progression of cervical adenocarcinoma.To judge CyclinD1 and P27 may be simple effective molecule marker that has the extremely significance to forecast prognosis through research.Materials and methods56 cases of cervical adenocarcinoma,30 cases of cervical glandular intraepithelial neoplasia and 24 cases of normal cervical glandular epithelium tissue from the First affiliated hospital of Zhengzhou University,the third affiliated hospital of Zhengzhou University and the Zhengzhou women and children's hospital.56 cases of cervical adenocarcinoma include 15 cases of well differentiated,21 cases of moderately differentiated and 20 cases of poorly differentiated.24 cases of normal cervical glandular epithelium tissue come from patient of total hysterectomy for hysteromyoma.After slicing 3μm to all cases,the HE stain.All cases were identified by two pathologists.To analysis its clinical material include of the clinical stage, lymphatic metastasis.The expression of CyclinD1 and P27 protein were detected in 56 cases of cervical adenocarcinoma,30 cases of cervical glandular intraepithelial neoplasia and 24 cases of normal cervical glandular epithelium tissue by immunohistochemistry.To analyze the pathogenesis of cervical adenocarcinoma.To discussion the relationship bewteen the expression of CyclinD1 and P27 and the pathological grade,the clinical stage, lymphatic metastasis of cervical adenocarcinoma.All the data were analyzed by SPSS11.5 statistical package,the count information calculated the positive rate.The comparision of positive rate uses the Chi-square test,the mean of more than two groups uses R×C Chi-square test,The relation of two variable are analyzed by Spearman's correlation analysis.The level of significant difference wasα=0.05Results1.The positive expression rate of CyclinD1 was respectively 62.50%,26.67%,and 0.00%in cervical adenocarcinoma,cervical glandular intraepithelial neoplasia, normal cervical glandular epithelium tissue.The positive rate was significantly higher in cervical adenocarcinoma and cervical glandular intraepithelial neoplasia than that in normal cervical glandular epithelium tissue(P＜0.05),The positive rate was significantly higher in cervical adenocarcinoma than that in cervical glandular intraepithelial neoplasia,a significant difference between them(P＜0.05).2.The positive expression rate of CyclinD1 was respectively 26.67%,61.91%and 90.00%in cervical adenocarcinoma that well differentiated,moderately differentiated and poorly differentiated,a significant difference between them(P＜0.05)。The positive expression rate of CyclinD1 was respectively 48.00%,74.19%in cervical adenocarcinoma in clinicalⅠ～Ⅱissue andⅢ～Ⅳissue,the difference has the remarkable statistics significance(P＜0.05).The positive rate of the expression of CyclinD1 in the group with lymph node metastasis and in the group without lymph node metastasis was 77.78%,48.28%,a significant difference between them(P＜0.05).3.The positive expression rate of P27 was respectively 30.36%,53.33%and 91.67%in cervical adenocarcinoma,cervical glandular intraepithelial neoplasia, normal cervical glandular epithelium tissue.The positive rate was significantly lower in cervical adenocarcinoma and cervical glandular intraepithelial neoplasia than that in normal cervical glandular epithelium tissue,a significant difference between them (P＜0.05).4.The positive expression rate of P27 was respectively 66.67%,28.57%and 5.00%in cervical adenocarcinoma that well differentiated,moderately differentiated and poorly differentiated,a significant difference between them(P＜0.05).The positive expression rate of P27 was respectively 51.72%,19.36%in cervical adenocarcinoma in clinicalⅠ～Ⅱissue andⅢ～Ⅳissue,the difference has the remarkable statistics a significance(P＜0.05) The positive rate of the expression of P27 in the group with lymph node metastasis and in the group without lymph node metastasis was14.81%,44.83%,a significant difference between them(P＜0.05)5.56 cases of cervical adenocarcinoma,5 cases are positive of CyclinD1 and P27,9 cases are negative,30 cases of CyclinD1 are positive and P27 are negative,12 cases of CyclinD1 are negative and P27 are positive.Cervical adenocarcinoma correlative analysis showed the expression of CyclinD1 has a negative relationship with the expression of P27(r_s=-0.451,P＜0.05).Conclusions1.Generation and development of cervical adenocarcinoma is closely correlated to positive expression of CyclinD1 and negative expression of P27.2.Abnormal expression of CyclinD1 and P27 is correlated to the histological grade,clinical stage and lymph node metastasis in cervical adenocarcinoma. Expression of CyclinD1 and P27 may be help judgement prognosis of cervical adenocarcinoma.3.The expression of CyclinD1 and P27 was reverse correlated.CyclinD1 and P27 may be have synergistic in development and progression of cervical adenocarcinoma. |
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