The Changing Of Expression Of Phosphorylated ERK1/2and MMP-9in Rats With Asthma And The Effect Of Budesonide On The Expression

Objectives:To study the expression of phosphorylated extracellular signal regulating kinase1/2(ERK1/2) and matrix metalloproteinase-9(MMP-9) in asthmatic rat lung tissue,the role of ERK signaling pathways and MMP-9in asthma airway remodeling,therelationship between each other and the effect of budesonide on the expression.MethodsThis experiment in4~6weeks SD rats as experimental objects,30rats wererandomly divided into normal control group(control group),Asthma model group(asthma group),budesonide intervention group (BUD group),10in each group. GroupB and group C were given OVA(ovalbumin) by intraperitoneal injection on day1, day8.Asthma group and BUD in day1, day8, respectively give ovalbumin(ovalbumin and OVA) by intraperitoneal injection of sensitization liquid sensitization,on day15,the rats were challenged by OVA in a container with not completelyairtight.then the rats was challenged by OVA form day15,30minutes ever day,thenext day1time,a total of eight weeks. The BUD group was teeated with1mgbudesonide befor challenged by OVA..Lung tissue were cellected in24th after the lastchallenged. To observe the rat lung tissue pathological changes,and detect theexpression of the p-ERK1/2and MMP-9in lung tissue by immunohistochemistry.After P-ERK1/2and MMP-9has been completed.At400times highmagnification, randomly selecting five horizons from each section, testing the IOD(Integrated option density) value of the p-ERK1/2and MMP-9of the lung tissue bythe Image Pro Plus Image analysis software,taking the average value as therepresentative of each slice.Result1. Lung tissue pathology change:HE staining indicate that in the control groupnormal structure of small airway and alveolar structure,in the asthma group airway epithelial cells necrosis and denudation,airway incrassation,inflammatory cellinfiltration around airway and vessels,in BUD group,the above changes were lighterthan the asthma group.2. To detected the expression of the p-ERK1/2and MMP-9with immuno-histochemistry.2.1The comparison of p-ERK1/2expression in ench group lung tissue: Compardwith control group,the expression of P-ERK1/2in asthma group and BUD group weresignificantly increased(P＜0.01).Compared with asthma group,the expression ofP-ERK1/2in BUD group were decreased significantly(P＜0.01),but was higher thancontrol group.2.2The comparison of MMP-9expression in ench group lung tissue:Compardwith control group,the expression of MMP-9in asthma group and BUD group weresignificantly increased(P＜0.01).Compared with asthma group,the expression ofMMP-9in BUD group were significantly decreased (P＜0.01),but was higher thancontrol group.3. The expression of p-ERK1/2was positively correlated with MMP-9inairway and lung tissue.Conclusion：1. The experiment show that p-ERK1/2and MMP-9play an important role inasthma airway remodeling,the expression of p-ERK1/2and MMP-9can be used as areference indicator of airway remodeling.2. The expression of p-ERK1/2was positively correlated with MMP-9in airwayand lung tissue,which show that they involved in the asthma pathogenesy together.