| Objective: Using the mice, sensitized with ovalbumin via introperitonealinjection and challenged with ovalbumin inhalation, to observe the expressionof IL-4, IFN-γ, TGF-β1, T-AOC and NO in lung tissue and the amount ofcollagen deposition around the bronchus, to investigate the effects and possiblemechanism of rhodiola combined with budesonide used on the airwayinflammation and airway remodeling and oxidative stress. Methods: Fifty-sixC57bL/6mice were randomly divided into seven groups, the control group(group A), the model group (group B), the budesonide group (group C),low-dose rhodiola group (group D), high-dose rhodiola group (group E),low-dose rhodiola combined with budesonide group (group F), high-doserhodiola combined with budesonide group (group G). asthma mice models weresensitized and challenged with ovalbumin. Budesonide was inhaled andrhodiola was administered intragastrically. We observed the characters ofclinical manifestations in mice. Liu’s stain method to analyze the number ofinflammatory cells in bronchoalveolar lavage fluid (BALF). The concentrationof TGF-β1, IL-4and IFN-γ in BALF was quantified by ELISA. To observe theairway inflammation and the amount of collagen deposition around thebronchus by HE and Masson. The expression of TGF-β1, IL-4and IFN-γ inlung tissue was detected by immunohistochemistry. The activity of NO was detected by total nitric oxide assay kit and the concentration of T-AOC wasdetected by total antioxidant capacity assay kit (ABTS). Results:1. Comparedwith group A, the total cells and the eosinophils in BALF of group B weresignificant increased (P<0.05), after treatment, the total cells and theeosinophils in BALF were all decreased in group C, group D, group E, group Fand group G, the high-dose rhodiola group was decreased more than that of thelow-dose rhodiola group, there was statistic significance between them (allP<0.05), the most significant decreased group was group G.2. Compared withgroup A (27.46±5.82,69.32±7.39) in BALF, The concentration of IL-4andTGF-β1in group B (259.16±4.27,160.49±6.41) was increased obviously(P<0.05), but after treatment, the concentration of IL-4and TGF-β1in group C(84.14±5.21,97.86±5.37), group D (147.53±3.59,113.13±6.28), group E(135.36±3.34,104.27±5.14), group F (78.38±6.04,91.32±7.03) and group G(73.14±2.93,85.24±7.08) were obviously lower than that of group B, there wasstatistic significance between them (all P<0.05). The most significant decreasedgroup was the high-dose rhodiola combined with budesonide group. Comparedwith group A (187.94±10.54), the concentration of IFN-γ in group B (72.53±9.43) was obviously decreased (P<0.05). After treatment, the group C(122.58±12.61), group D (92.7±11.39), group E (103.4±9.72), group F(126.37±10.39) and group G (130.29±8.22) were significantly higher than thatof group B, there was statistic significance between them (all P<0.05). Themost significant increased group was the high-dose rhodiola combined with budesonide group.3.(1) hematoxylin-eosin staining: The control group had notany inflammation changes, the model group had very severe inflammation,after treatment, the inflammation cells were all decreased in group C, group D,group E, group F and group G, the high-dose rhodiola group was decreasedmore than that of the low-dose rhodiola group, the most significant decreasedgroup was group G.(2). Masson staining: Compared with group A (5.4±1.3),The Wcol/Pbm in group B (32.8±1.1) was increased obviously (P<0.05). Aftertreatment, the group C (10.7±2.5), group D (11.9±2.6), group E (11.0±2.4),group F (9.9±3.2) and group G (8.7±1.9) were obviously lower than that ofgroup B (5.4±0.3), the most significant decreased group was group G, there wasstatistic significance between them (all P<0.05).4. By immunohistochemistry,the expression of IL-4and TGF-β1in lung tissue of group B (0.335±0.017,0.417±0.008) was obviously higher than that of group A (0.039±0.006,0.168±0.003)(P<0.05), but after treatment, the level of IL-4and TGF-β1ingroup C (0.130±0.003,0.238±0.004), group D (0.156±0.009,0.298±0.017),group E (0.143±0.005,0.271±0.013), group F (0.126±0.013,0.227±0.015) andgroup G (0.114±0.002,0.213±0.009) were obviously lower than that of group B(P<0.05). The most significant decreased group was the high-dose rhodiolacombined with budesonide group. Compared with group A (0.314±0.027), theexpression of IFN-γ in group B (0.093±0.009) was obviously decreased(0.314±0.027)(P<0.05). After treatment, the group C (0.276±0.005), group D(0.238±0.012), group E (0.249±0.004),group F (0.282±0.014) and group G (0.288±0.003) were significantly higher than that of model group (P<0.05), themost significant increased group was the high-dose rhodiola combined withbudesonide group, there was statistic significance between them (all P<0.05).5.Compared with group A (0.328±0.124,1.030±0.021), the activity of NO ingroup B (1.235±0.084,0.475±0.023) was obviously increased (P<0.05), and theexpression of T-AOC was decreased (P<0.05). After treatment, the activity ofNO in group C (0.764±0.007), group D (0.892±0.156), group E (0.813±0.005),group F (0.715±0.016), group G (0.669±0.172) were significantly decreased(P<0.05), but the expression of T-AOC in group C (0.750±0.106), group D(0.502±0.202), group E (0.580±0.219), group F (0.780±0.061), group G(0.803±0.110) were increased, there was statistic significance between them (allP<0.05). Conclusion:1. Asthma mice model was successfully established bysensitized with ovalbumin via introperitoneal injection and challenged withovalbumin inhalation.2. The rhodiola and budesonide could inhibit theexpression of IL-4, TGF-β1, NO and increase the level of IFN-γ, T-AOCrespectively in lung tissue. But the effects of budesonide were better.3.Therhodiola could inhibit the airway inflammation,airway remodeling andoxidative stress in asthma mice. The effects of rhodiola were presented in adose-dependent manner.4. There was a synergistic interaction between rhodiolaand budesonide on the inhibitory expression of TGF-β1and the regulation ofthe balance of IL-4/IFN-γ, NO/T-AOC. |
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