NF-κB Signal Pathway And Downstream MicroRNA Activation And Expression In Xenografted Cervical Cancer And Its Adjacent Tissues

Objective：To investigate the expression of miR-15b and miR-16as well as the status ofNF-κB signaling in xenografted cervical cancer and its adjacent tissues, which willprovide new insight for the early diagnosis and treatment of cervical cancer.Methods：Mice cervical cancer U14cells suspension injected in Kunming mice abdomento establish ascites tumor model. After three generation, using ascites cells toestablish tumor-bearing mouse model in right armpit of thirty Kunming mice. Whenthe xenografts grown to1.5cm×1.5cm（about15days）, xenografted cancer and itsadjacent tissues, normal fat tissues were collected respectively. The expression ofmiR-15b and miR-16were detected by real-time PCR. The expression andsublocation of NF-κB(p65) was analyzed by immunohistochemistry technology.Results：1. Mice cervical cancer U14cells suspension injected in Kunming miceabdomen，after two weeks, the modle of ascites tumor are successful. Then usingascites cells to establish subcutaneously tumor-bearing mouse model. After twoweeks, the rate of tumor are100%. HE staining of the xenograft indicated that themodel is successful.2. Real-time PCR analysis showed that the miR-15b levels in the cancer are32.21±3.67, which significant higher than its adjacent tissues25.16±1.86and normalfat tissue1.00(P<0.05)，adjacent tissues also significant higher than normal fat tissue(P<0.05); miR-16levels in the cancer are28.63±2.34,which significant higher thanadjacent tissues22.16±1.76and normal fat tissue1.00(P<0.05)，adjacent tissues alsosignificant higher than normal fat tissue (P<0.05).3. Immunohistochemistry analysis showed that NF-κB(p65) was mainlylocalized in cytoplasm of normal fat tissues, whereas in the cancer tissues andadjacent non-tumorous tissues， NF-κB(p65) translocated into the nucleus. The expression of NF-κB(p65) in cancer tissues was significant higher than its adjacenttissues and normal fat tissue (P<0.05), adjacent tissues also significant higher thannormal fat tissue (P<0.05).Conclusion：1. NF-κB signal pathway was activated both in the cervical cancer tissues and itsadjacent tissues.2. NF-κB regulated miR-15b and miR-16were upregulated significantly in bothcancer tissues and its adjacent tissues.3. NF-κB signal pathway and its regulated miR-15b and miR-16were involvedin early molecular events of cervical cancer.