An Experimental Study Of Carisolv Chemomechnical Caries Removal Method Action On Caries

Dental caries is a common and frequently-occurring disease. With thedeepening understanding of the biological basis of dental caries and thedevelopment of adhesive materials, it has been put forward by modern oralmedical: the more normal tooth structure should be preserved as far aspossible in the process of treatment. As Carisolv chemomechanical cariesremoval method selectively removed the infected carious dentin, it is aminimally invasive preparation technology. In our research, we hadevaluated that Carisolv gel affected on the physical and chemical propertiesof healthy dentin by comparing microhardness and the ratio of Calcium andPhosphorus before and after Carisolv action on the healthy dentin, andcompared microhardness, the ratio of calcium and phosphorus, themicroleakage of the bonding interface between Carisolv and conventionalbur methods. We aimed to seek a selection clinical method to cariesremoval of security, comfortable and saving more dentin.Part One: Microhardness and ratio of calcium and phosphorus of the healthy dentin after Carisolv action on the surfaceMethods:10healthy primary molars and10permanent molars withoutdentinal caries were used. All the teeth were sectioned longitudinally at thecenter of occlusal surface along the mesial-distal direction, the buccal orlingual enamel of all the samples were wiped off with diamond burs down to1mm beneath enamel-dentinal junction. The exposed dentin surface waspolished by grit silicon carbide paper. Carisolv was applied on the healthydentin surface for one part of teeth, and the others were used as the controlgroup without any further processing. Microhardness was tested using amicrohardness tester and the ratio of calcium and Phosphorus was measuredusing energy dispersive analysis of x-rays（EDAX）. Results: there was nosignificant difference after Carisolv action on the surface, both for primaryand permanent teeth (P>0.05). Conclusion: Carisolv didn’t affect thephysical and chemical properties of healthy dentin.Part Two: Microhardness and ratio of calcium and phosphorus ofdentinal surface after Carisolv chemomechanical caries removalMethods:10primary molars and10permanent molars with dentinalcaries, but not involving the pulp were collected. All the teeth weresectioned into two parts along the center of the cavity. Carisolv system wascompared with slow-speed burs in eliminating caries. Then microhardnesstest and EDAX were performed on the floor of the cavity. Results: the resultsshowed no significant difference between the two methods, whether for primary or permanent teeth (P>0.05). But there was significant differencewhen caries-affected dentin was compared with healthy dentin in thepermanent group (P<0.05). Conclusion: Carisolv system could removecaries effectivly as rotary instrument.Part Three: Microleakage of dentinal bonding interface after Carisolvchemomechanical caries removalMethods:30primary molars and30permanent molars with dentinalcaries but not involving the pulp were used. All the teeth were randomlydivided into two groups, Carisolv system was compared with slow-speedburs in eliminating caries. After carious dentin was removed, the excavateddentin surfaces in each group were restored with composite resin Z250, theteeth were immersed in artificial saliva under constant temperature at37℃,and5teeth in each group were selected by randomly in period of24h,7d,3m. Specimens were immersed into0.1%Rhodamine B for24h, then cut into1mm thickness slices and the amount of microleakage was measured by1aser scanning confocal microscope(LSCM). Results: there was nosignificant difference for the two methods in the three periods, both forprimary and permanent teeth (P>0.05), and there was no significantdifference between each group over time by longitudinal observation also.Conclusion: Carisolv chemomechanical caries removal could obtain goodedge closeness.