Analysis Of The Single Nueleotide Polymorphisms Augmenter Of Liver Regeneration Promoter Region With ACLF

objective:1)To analyze value of clinical the patients suffering HBV infectionthat had different liver damaged using ELLISA method.2)To obtained allelefrequency the SNP site of ALR gene promoter in Han population.3)To screenedSNP site of ALR gene promoter with ACLF.4）To detected ALR serumconcentration difference in SNPs among the different genotypes of ACLF.Methods:The patients HBV-related liver diseases more than368in-patient or the clinicalwere collected from2010to2013,including CHB(n=110)ACLF(n=138) andhealthy blood donors(n=120). Each groups collected blood were distributed intoblood cells and plasma frozen at-20℃to stored.1)ELLISA: To selectedpatients serum of each group and in-patient serum of ACLF.To observed Elisavalues each group.2)Blood DNA extraction: EDTA anticoagulated bloodsamples were placed in a37℃incubator rapidly dissolved. extracted genomeDNA using the QiAamp DNABlood.Concentration and purity were detectedwith spectrophotometer.3)Electrophoresis and PCR: to extracted and detectedsample of DNA put into1.5%agarose gel were subjected to electrophoresis inthe elcectrophoresis buffer, finally the results observed in the ultraviolettransillumination. Followed by PCR products were send to invitrogen company to sequence.4). We determined to judge genetype and to analysis statisticallysignificant different.To analysis correlation between genetype and ALRexpression；To screened SNP site of ALR gene promoter with ACLF。Results:1.Serum ALR level was higher in ACLF than in normal control(2.58±1.67vs.0.72±0.29,p<0.01). Bewteen serum ALR level in CHB andnormal control is not not statistically significant（0.74±0.46vs0.72±0.29，P=0.813）. Serum ALR level of patients with ACLF was more significant insurvival group than in dead group.(7.78±1.75vs.2.10±1.50.t=7.479,p<0.01).Logistic regression analysis that there are P=0.000,OR value0.003，95%（0.000,0.034）. High ALR level in serum in ACLF may mean a goodprognosis.2. The ALR gene promoter polymorphisms(-847A/G,-393A/G,-80G/A)weresuccessfully genotyped in all group.The genotype were not statisticallysignificant in ACLF patients, normal control and CHB(P>0.05).However, The-847A/G,-393A/G,-80G/A genotype were statistically significant in ACLFpatients than in normal control, Logistic regression analysis and stratificationanalysis with adjustment for age and sex indicated that the polymorphisms of-80G/A were associated with susceptibility to ACLF(P<0.05).-80G/A in theALR gene promoter was associated with an increased susceptibility to ACLF(OR=1.788,95%CI1.095,3.263,P=0.028)3. Patients of ACLF was notstatistically significant to ALR value in different genotype (P>0.05).Conclusion: Based on population genetic association study and genetic functional analysis of the cadidate genes,our study emphasizes the importanceof ALR in the pathophysiology of ACLF on the population level, Serum ALRlevel of patients with ACLF was more higher than other groups so that ALRlevel in serum may indicate hepatocyte proliferation or liver regeneration. HighALR level in serum in ACLF may mean a good prognosis. ACLF related SNPsites-847A/G,-393A/G,-80G/A.-80G/A sites in the ALR gene promoter wasassociated with an increased susceptibility to ACLF. Patients of ACLF was notstatistically significant to ALR value in different genotype.