The Effects Of Zhaqi Jiangtang Decoction On The Expression Of TGF-β₁,VEGF And Nephrin In The Kidney Of Rats With Diabetic Nephropathy

Objective To investigate the effects of Zhaqi jiangtang Decoctionon the expression of TGF-β₁、VEGF and nephrin in kidney of rats withdiabetic nephropathy,and the possible mechanisms of its renal protection.Methods60SD rats,8weeks old,200±20g, a week’s adaptivefeeding.Then the rats were randomly selected8as the normal group（N，n＝8）, the rest of them injected STZ to establish the model rats of diabetic.After4weeks, the model rats of DN were divided by random numbertable into5groups: Model group（M，n＝10）, Glibenclamide group（G，n＝10）, Zhaqi jiangtang Decoction high-dose group（ZG, n＝10）, Zhaqijiangtang Decoction mid-dose group （ZZ, n＝10）, Zhaqi jiangtangDecoction low-dose group（ZD, n＝10）. Then the relevant drugs weregiven intragastrically to different groups respectively. At the end of10thweek, take Inner canthus blood to measured BUN, Scr by routineanalyse methods; take renal tissues to detect the pathological morphologychanges and ultrastructural changes; the expression of TGF-β₁was detected by immunohistochemical staining; the expression of VEGF wasdetected by immunofluorescence staining; the expression of nephrinmRNA and nephrin protein were determined by applying RT-PCR andWestern blot.Results In model group, BUN, Scr in blood were higher than thenormal group（P＜0.05）, renal tissue pathological morphology changesand ultrastructural changes were significant, the expression of TGF-β,VEGF was significantly higher than the Normal group（P﹤0.01）, and theexpression of nephrin mRNA and protein was significantly lower than theNormal group（P﹤0.01）. Compare with model group, other groupsimproved significantly, especially in Zhaqi jiangtang Decoction high-dose group and Glibenclamide group （P <0.05）, in which the blood BUN,Scr were decreased, the expression of TGF-β₁, VEGF weredown-regulated, the expression of nephrin mRNA and nephrin proteinwere increased.Conclusion Zhaqi jiangtang Decoction has a protective effect onthe kidney of rats with diabetic nephropathy, and the mechanisms may bethrough down-regulation the expression of TGF-β1, VEGF, up-regulatethe expression of nephrin mRNA and nephrin protein to reducegeneration and precipitation of glomerular mesangial matrix, andreduce podocyte injury.