| Background and ObjectiveContainer as a special loading container, improve handling efficiency, shorten the berthing time, saving handling costs, reduce transportation costs, has confined moisturizing, insulation characteristics, making it and there is a great deal of public health and environmental health risks. Firstly, the spread of medical vector carriers. Secondly, medical vectors of growth, reproduction places. Thirdly, illegal occult metastasis pollutants tools. Fourthly, become a source of contamination of pathogenic microorganisms. In addition, the container itself is part of the structure is a plant product category, with the transport of long-term use and wear it in the structure and materials have to carry the soil, plant and animal residues, plants against diseases, pests, weeds and other pests and other epidemic disease outbreaks and conditions of toxic and hazardous substances.In China, the General administration of quality supervision, inspection and quarantine, promulgated Codes for disinfection of entry-exit containers and inner cargo and Inspection rules for sanitary quarantine of entry-exit containers in2003and developed a series of standard procedures for the entry and exit of empty containers disinfection. However, most of these standards inside or rely on artificial means to carry out disinfection work. Including, firstly the tow truck driver needs to container haulage to the gate under the inspection and quarantine disinfection area, staff armed drug into container box, and then close the door fumigation. When the disinfection is completed, staff need to open the door, and detected inside the gas residues reached only after a job allowable concentration when the procedures in fumigation. Secondly, the tow truck driver needs to container haulage to the gate under the inspection and quarantine disinfection area staff armed with disinfectant spray equipment, disinfection treatment, on the outer surface and inner surface of the container when the procedures in spray disinfection.The traditional manual mode of empty containers for disinfection, although you can reach a disinfection effect, but the pattern is insufficient. Such as, firstly, disinfection process due to personal reasons, resulting in its medication uneven, is not complete. When in disinfection, staff existence of empty container health processing do not pay attention not seriously attitudes, neglect disinfected operating requirements, in accordance with the long-term practice of disinfection, lead work, just to cope with the processing, sloppy empty container things, not be able to do to conduct a comprehensive and thorough disinfection. Secondly, when in manual disinfection, it is difficult to avoid harmful substances as well as disinfectants have adverse effects on human health when in artificial disinfection. When disinfecting the empty container, staffs often require fumigation of disinfectants, medicines, medical biological vectors, pathogenic organisms and toxic chemicals in close contact and this will have adverse impact on staff health. Thirdly, disinfection time is too long and low efficiency. Artificial disinfection, time is often needed in about10minutes, if found inside of empty containers subject to inspection and quarantine objects, especially for problems TEUs need by sampling, report, health and epidemic prevention deal with this series, and this process the time it takes to reach more than half an hour and it would greatly reduce the efficiency of the inspection and quarantine work. Fourthly, Increase the cost of consumption of port enterprises. The traditional manual disinfection mode, port enterprises often need to hire a certain number of staff dedicated to the area of empty container disinfection, and carve out a place in the port area, which tend to increase the cost and resource consumption on a day operations of the port enterprises, not conducive to the development of the enterprise.Because of the way traditional manual shortcomings, in the individual conditions of the seaport, start enable automatic disinfection of containers and processing equipment. Through the introduction of the automatic disinfection equipment, improve the working efficiency and the flow. However, the device still has its shortcomings, including:firstly, the stability of the equipment needs to be improved. Secondly, is not completely automated, still use manual mode.In view of the current manual disinfection mode and old semi-automatic disinfection of the equipment deficiencies of, after a long period of practice and exploration, we have developed a new generation of fully automated rapid sterilization equipment. In order to further assess the actual effectiveness of devices, seaport gates treatment capacity and improve the quality and efficiency of research on disinfection efficacy of devices. Through the test evaluation of the effect of disinfection of empty containers, assess the working status of equipment; provide the scientific basis for further optimization and improvement and perfection.Methods1. Respectively hold freeze-dried strains of Escherichia coli, Staphylococcus aureus and Candida albicans saves tubes, sterile operation, inoculating loop strain picked a small amount taken into test tube filled with10ml brain heart infusion, after slight oscillation, reset training from18h to24h at36℃±1℃incubator. Inoculation loop pick takes first suspension cultured bacteria, streak inoculation on the medium for isolation of different, at36℃±1℃incubator training from18h to24h. Inoculation loop pick typical colonies take these second generation cultured, crossed on the nutrient agar plate inoculation, at36℃±1℃incubator training18h to24h, third generation of culture. Finally ring pick taken above the third generation of nutrient agar plate inoculation of fresh culture, added to the standard maxwell-turbidity tube, turbid meter for determination of concentration of the bacterial suspension are required. Experimental and quantitative identification of neutralizing agents for disinfectant germicidal test suspension in laboratory bio-safety cabinets.2. Suspensions of Escherichia coli bacteria and Candida albicans suspension respectively in no bacteria bovine serum albumin, proportion to them and mix with1:1, dubbed respectively and50%bovine serum-containing bacteria suspension. Set of role time within for quantitative hanging liquid sterilization test when the composite quarter ammonium salt of concentration for50mg/L, calculation killing log values. Acid alkali regulation agent respectively will disinfectant of pH value regulation for4groups and set of role time within respectively for quantitative hanging liquid sterilization test when the composite quarter ammonium salt of concentration for50mg/L, calculation killing log values. Uses thermostat water bath pot regulation temperature in test range within. Set the time quantitative germicidal test suspension respectively when the compound quaternary ammonium salt in a concentration of50mg/L, and calculated killing log values.3. Before sampling, we planning to set up four sampling points before and after disinfection, and sampling by the microbial air-sampler inside of empty containers at the seaport field. After all sampling, calculate the bacterial content in the air and the killing rate, and observation of air disinfection for equipment to internal empty containers.4. Before the test start, we paste some bacterial carriers with preparing in laboratory to empty containers inside and outside surfaces. We would sampling before and disinfection, and calculate the killing log values when all sampling finished. Observation of bacterial carrier’s disinfection for equipment to empty container surface. After simulated field test, would sampling before and disinfection of inside empty container surface at the seaport field and calculate the natural bacteria bacterial content and killing rate for empty container surface. Observation of natural bacteria’s disinfection for equipment to empty container surface.Result1. It showed that the average killing log of Escherichia coli and Staphylococcus aureus were above5.0when the quaternary ammonium compounds disinfectant containing100mg/L active ingredient contact with1minutes; the average killing log of Candida albicans was above5.0when the quaternary ammonium compounds disinfectant containing100mg/L active ingredient contact with5minutes.2. The germicidal efficacy was lowed with organic substance, and the efficacy was enhanced with the up of temperature and pH when the quaternary ammonium compounds disinfectant containing50mg/L active ingredient.3. The new quick disinfection equipment produced an average killing rate of3trials were85.51%,88.98%and85.31%for air microbial aerosols in empty container.4. Average killing log value of5.34, respectively for126bacterial carriers of E. coli for the disinfection equipment spray disinfection to empty container’s outer and inner surface in simulated field test. There are113carriers were be qualified and13carriers were be unqualified in126bacterial carriers affixed to the empty container surface, and the overall pass rate was89.68%according to killing log value and the standard with more than or equal to5and smaller or equal to5.Average killing log value of5.23, respectively for126bacterial carriers of Staphylococcus aureus for the disinfection equipment spray disinfection to empty container’s outer and inner surface in simulated field test. There are112carriers were be qualified and14carriers were be unqualified in126bacterial carriers affixed to the empty container surface, and the overall pass rate was88.98%according to killing log value and the standard with be more than or equal to5and be smaller or equal to5.Average killing rate of95.94%, respectively for40sampling points for the disinfection equipment spray disinfection to empty container’s inner surface in field test. There are29samplers were be qualified and11samplers were be unqualified in40samplers to the empty container surface, and the overall pass rate was72.5%according to killing rate and the standard with more than or equal to95%and smaller or equal to95%.Conclusion1. The seaport gates fast disinfection equipment used disinfectant good killing effect on the multiplication of bacterial and fungal propagules can be used in daily disinfection of empty containers at the port.2. The seaport gates fast disinfection equipment used disinfectant bactericidal effect will be the impact of organic matter, temperature and pH. Daily disinfection of empty containers to be adjusted according to the actual situation disinfection conditions of use of the equipment and technical indicators.3. The seaport gates fast disinfection equipment air disinfection inside of empty containers, which have a good effect on killing, but there is still a gap compared with the relevant provisions of the need to improve the effect of microbial disinfection equipment air.4. The seaport gates fast disinfection equipment disinfectant spray on the inner and outer surfaces of empty containers, the effect of the inside of the inside surface of the container empty than other poor surface equipment needed for the factors affecting the device atomization device perfect. |
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