Expression Of FOXO1in Various Types Of Endometrial Hyperplasia And Endometrial Adenocarcinoma

Objective:1、 To detect the expression of FOXO1in normal proliferative,simplehyperplasia,complex hyperplasia,atypical hyperplasia endometrium andendometrial adenocarcinoma specimens. To observe the relationship betweenFOXO1and endometrial adenocarcinoma clinicopathological factors,andexplore FOXO1significance in the process of development of endometrialadenocarcinoma lesions.2、 comparing the expression of FOXO1and HOXA10individually, andanalyzing its correlation and Possible role in various types of endometrium.Methods:To Detect the expression level of FOXO1and HOXA10protein in16cases ofnormal proliferative,16cases of simple hyperplasia,18cases of complexhyperplasia,20cases of atypical hyperplasia endometrium and54cases ofendometrial adenocarcinoma by using immunohistochemical method (SP).Results:1、FOXO1showed a strong expression of the normal endometrial hyperplasia andlesions endometrial tissue. In poorly differentiated endometrial FOXO1expressionlevels were significantly lower, the expression of FOXO1decreased gradually inthe endometrial glandular epithelial cell nuclei of normal hyperplasia, simplehyperplasia, complex hyperplasia; the expression in atypical endometrial hyperplasia and endometrial cancer glandular epithelial cell nuclei was significantlylower and the difference was statistically significant. In contrast, FOXO1wasstrong expression in atypical endometrial hyperplasia and well-differentiatedendometrial glandular epithelial cytoplasm, the expression In normal hyperplasia,simple hyperplasia, complex hyperplasia endometrial glandular epithelial cytoplasmwas significantly reduced（P<0.05）with gradually increasing trend. FOXO1expressed in various types of endometrial stromal cells in the nucleus with almostno expression in the cytoplasm, from the normal proliferative to endometrialadenocarcinoma, the expression level of FOXO1in the Stromal cells decreasedgradually, almost no expression in adenocarcinoma.2. The FOXO1expression in the different degree of differentiation of endometrialcancer are also different.The expression of FOXO1in well-differentiated andmoderately differentiated endometrial adenocarcinoma gland epithelial cell nuclei/Cytoplasm show no significantly different,while in poorly differentiatedendometrial adenocarcinoma was significantly reduced, and the difference wasstatistically significant (P <0.05).3、Compared with ER (+) PR (+) endometrial adenocarcinoma, the expression ofFOXO1in the ER (+) PR (-) adenoycarcinoma of the glandular epithelium nucleidecreased (P <0.05), and contrastly increased in the ER (+) PR (-)adenoycarcinoma (P <0.05).expression in the nucleus and cytoplasm of of theglandular epithelium in ER (-) PR (-) adenocarcinoma significantly reduced, thedifference was statistically significant (P <0.05).4、HOXA10is only expressed in the nucleus of normal endometrial hyperplasia andlesions endometrial glandular epithelial and stromal cells. From normal hyperplasia,simple hyperplasia to complex hyperplasia endometrial glandular epithelial cells,HOXA10expression showed a downward trend but with no statistical significance.Expression decreased dramatically, and was statistically significant (P <0.05) inatypical hyperplasia and endometrial adenocarcinoma.5、From normal proliferative endometrium to endometrial adenocarcinoma, FOXO1and HOXA10expression in endometrial performance parallel horizontal, the relevant statistical analysis showed that the expression of FoxO1and HOXA10arehighly correlated.Conclusion:Nuclear translocation of FOXO1in endometrial glandular epithelial cellspromotes the development of endometrial cancer， The downregulation ofHOXA10are related to the development of endometrial cancer。the FoxO1&HOXA10may interact with each other in endometrial glandular epithelial cellnuclei to participate in the development of endometrial adenocarcinoma.