The Preliminary Study Of HOXA10 Acetylation By PCAF In Regulating Human Endometrial Receptivity

Endometriosis (EMT) is one of the main reasons for the infertility in adult women. It has been reported that homeoboxa 10 (HOXA10) acts as a transcription factor during embryo implantation, but the regulatory mechanism of its biological function is still unclear. In a yeast two-hybrid screening experiment, we found that the acetyltransferase P300/CBP associated factor (PCAF) interacted with HOXA10, suggesting that PCAF might acetylate HOXA0 during the process in establishing endometrial receptivity.In this study, PCAF was identified as a HOXA10-interacting protein and HOXA10 associated with PCAF through its homo-box domain. PCAF inhibited HOXA10-mediated β3-integrin (ITGB3) transcription through its histone acetyltransferase (HAT) activity (P <0.05). Over-expression or knockdown of PCAF showed PCAF down regulated HOXA10-mediated ITGB3 protein expression by acetylating HOXA10 and significantly diminished HOXA10 mediated embryo adhesiveness in Ishikawa cells (P<0.05).We also found that, there was an aberrant high PCAF expression in the endometrium of women with endometriosis than that in normal controls (P<0.05).In summary, HOXA10 associates with and is acetylated by PCAF in Ishikawa cells and HOXA10-PCAF association impairs embryo implantation by inhibiting ITGB3 protein expression in endometrial epithelial cells.