Study On MicroRNA Expression Profile And Target Gene Prediction In Sporadic Parkinson’s Disease

Object: To explore the differential microRNA (miRNA) expression between thesporadic Parkinson’s disease (PD) with and without on-off and the control group. Followedby target gene prediction.Methods: We selected six sporadic PD patients, which were separated into two groupsaccording to whether with on-off, and three healthy control subjects matched in ethnicityand origins. Blood samples were collected and total RNAs were isolated. Expressions ofmiRNAs were detected by using microRNA microarray. Finally, the differentially expressedmiRNAs were chosen for target genes prediction.Results：The differentially expression of miRNAs has been found in the six sporadicPD patients compared to the healthy controls. The patients with on-off has greaterdifferential expression. There are nine miRNAs co-express between the on-offs and the noon-offs, which include the up-regulated miRNAs hsa-miR-589-3p and-miR-302e, and thedown-regulated miRNAs hsa-miR-5582-3p，-miR-4483，-miR-4481，-miR-4501，-miR-2113，-miR-493-3p and-miR-5001-3p. According to the results of gene prediction,LRRK2, which is now considered the most likely incidence of gene associated withsporadic PD, can be predicted by has-miR-4483. GIGYF2and EIF4G1can also bepredicted by those co-expressed miRNAs.Conclusions: MiRNAs may take part in the pathogenesis and process of sporadic PD.We believe that the specific differential miRNAs can be seen as new biomarkers in thediagnosis and therapy of sporadic PD.