The Effects Of Cytokines In Adipose Stem Cell-conditioned Medium On The Migration And Proliferation Of Skin Fibroblasts In Vitro

Background：Although adipose stem cell-conditioned medium (ASC-CM) has beendemonstrated the effect on promoting the cutaneous wound healing, the mechanismfor this response on the effector cells (like dermal fibroblast) during the processremains to be determined.Objective: The aim of this study was to determine the concentration and importanceof EGF, PDGF, VEGF, bFGF in ASC-CM on dermal fibroblast responses to woundincluding proliferation and migration.Methods: The protein microarray analysis was chosen to define the content of507cytokines in ASC-CM, including EGF, PDGF, VEGF and bFGF. In the further study,the tans-well assay and scrape-wound healing assay were chosen to analyze thefibroblast migration, and the CCK-8assay to the fibroblast proliferation.Results: Results showed that ASC-CM had a higher concentration of the fourcytokines. The proliferation of skin fibroblasts were significantly stimulated by bFGFand EGF, and the migration of skin fibroblasts were significantly accecelarated byVEGF, bFGF and PDGF in ASC-CM. However, the effect of ASC-CM on fibroblastproliferation and migration was better than single cytokine.Conclusion: These observations suggested that ASC-CM played an important role inthe cutaneous injury partly because of the cytokines inside which could promote theproliferation and migration of dermal fibroblasts. As a result, ASC-CM was moreadaptive than each single cytokine to be applied in promoting the wound healing.