Effect Of PAR1-CX43Signal Pathway On The Ability Of Nasopharyngeal Carcinoma Cells Migration And Invasion

Objective: To investigate protease-activated receptor1(Protease-activated receptor,PAR1)on the proliferation and invasion of nasopharyngeal carcinoma cells(CNE1-LMP1) and its mechanism.METHODS: We used MTT assay, Transwell invasion assay and wound-healing testto detect the proliferation, invasion and migration of CNE1-LMP1treated bySFLLRN and SCH79797. Then, the expression of connexin43were detected usingWestern blot and RT-PCR.Results: We found PAR1synthetic activating peptide SFLLRN could promoteCNE1-LMP1cell proliferation, migration and invasion, and CX43mRNA and proteinwere significantly reduced. Whereas, PAR1antagonist SCH79797could inhibitCNE1-LMP1cell proliferation, migration, and in vitro invasion ability, and CX43mRNA and protein were significantly increased.Conclusion: PAR1may contribute to proliferation and invasion of CNE1-LMP1viaregulation of Connexin43. Connexin43acts as a tumor suppressor gene with loss ofCx43contributing to metastasis, which may serve as a potential therapeutic target forSuppress nasopharyngeal carcionma progression.