Quantum Dots Immunofluorescence Method In The Diagnosis Of Non-hodgkin’s Lymphoma In Children

Objective Children’s lymphoma is a malignant blood diseases in children afterleukemia, so early diagnosis plays a very important role in its treatment and prognosis.Quantum dots as a new type of fluorescent nanoparticles with many advantages hasbeen developed in recent years, such as, the excitation spectrum of wide andcontinuous, narrow and symmetric emission spectra, high fluorescence intensity,photochemical stability, light bleaching. In this study, the application of quantum dots(QDs) immunofluorescence non-Hodgkin’s lymphoma in paraffin-embedded tissuesections simultaneously detects both antigens expression and immune enzymehistochemical methods to explore based on quantum dots diagnosis rapid specificityand superiority, to be extended to the diagnosis of other diseases.Methods (1) Under the mild conditions, water-soluble of thioglycolic acid quantumdot was prepared out in the aqueous phase, and the different emission wavelengths ofthe quantum dot (525nm,605nm) were used to label streptavidin and prime compositeobject (QDs-SA) as the basis for the immune fluorescencetechnology, and detectT-cell lymphoblastic lymphoma, diffuse large B-cell lymphoma cases the surface ofthe tissue TDT, co-expression of CD3and CD19, CD20two antigens;(2) Enzymeimmunoassay technology was used to mark the order on serial sectionsantigen, andcompare the two methods antigen localization effects and assessment of the strengthand stability of the quantum dot optical immunofluorescence.Results The optical properties of quantum dots with thioglycolic acid modified QDs-SA composite were scarcely changed, and the experiment did not affect theorganization of cellular morphologic feature. Under the fluorescence microscope,almost all T-cell lymphoblastic lymphoma (17/20) has the co-expression of TdT、CD3antigen, and the majority of diffuse large B-cell lymphoma (11/20) has theco-expression of CD19, CD20. This is entirely consistent with immune enzymehistochemistry test results positioning the former positive signal stronger, higheroptical stability, and no obvious fluorescent particle aggregation imaging, imageclearer.Conclusion After surface modification of the quantum dots has a better tissuecompatibility, and reduces the toxicity of its surface. The use of quantum dotsimmunofluorescence assay can detect tissue expression of two antigens in paraffinsections, and has a high degree of consistency with immune enzyme histochemicalmethods. Due to the enzyme immunoassay method can mark only one antigens onone slice, the former can significantly save the amount of specimen and has higherpositive signal and stronger optical stability. Therefore, the quantum dots as a newtype of fluorescent probe, showing the feasibility and superiority，is expected tobecome a new auxiliary examination method in the pathological diagnosis.