Simvastatin-loaded Biological Ca/P Coated Titanium Plate On The Effect Of Bone Regeneration

Part1Simvastatin-loaded biological Ca/P coated Titanium plate on promoted the effect of osteogenic differentiation of mesenchymal stem cellsObjective This study aimed to investigate the effect of To study simvastatin-loaded biological Ca/P coated Titanium plate simvastatin can promote on the osteogenic differentiation of mesenchymal stem cells.Materials and methods Acid-base heat titanium plate and then were immersed in SBF bionic solution containing simvastatin and simvastatin-loaded biological Ca/P coated Titanium plate was fabricated. Produced containing simvastatin biological calcium phosphate coating of titanium plate, bionic calcium phosphate coating of titanium plates and pure titanium plates is set to the served as control. Mesenchymal stem cells grown were cultured in the titanium plate, calcium phosphate coating of titanium plates and simvastatin calcium phosphate coated titanium plates. After culturing, cultured for7days to14days, alkaline phosphatase expression of MSCs was the detection of alkaline phosphatase expression.Results MSCs cultured in Simvastatin-loaded biological Ca/P coated Titanium plate processing MSCs had the highest expression of alkaline phosphatase, followed by biological calcium phosphate coating of titanium plate, pure titanium plate (P<0.05). expression of at least.Conclusions This method can get c Simvastatin-loaded biological Ca/P coated Titanium plateontained with simvastatin biological biomimetic calcium phosphate coating of titanium plates, and. simvastatin can be slowly released and the promotioned the osteogenic differentiation of mesenchymal stem cells of MSCs in bone differentiation. Part2Simvastatin-loaded biological Ca/P coated Titanium rods on the effect of promoted bone regeneration and integration in vivoObjective Simvastatin-loaded biological Ca/P coated Titanium plate can promote the ingrowth of new bone, increase bone connecting surface adhesionMaterials and methods Simvastatin-loaded biological Ca/P coated Titanium plate was fabricated.Production of simvastatin biological biomimetic calcium phosphate coated titanium rods in the same way. bone defects (2mm diameter) were made in the rabbit femoral shaft of rabbits and Simvastatin-loaded biological Ca/P coated Titanium rod. do three2mm diameter bone defect, then implanted into the contained the simvastatin biological biomimetic calcium phosphate coating of titanium rods, the bionic calcium phosphate coated titanium rod and pure titanium rod.4weeks and8weeks after surgery, samples were detected by histology, scanning electron microscopy and spectroscopy detection of bone regeneration.Results the Simvastatin-loaded biological Ca/P coated Titanium rod Containing simvastatin bionic calcium phosphate coated titanium rod group had, the most bone tissue ingrowth. Scanning electron microscopy and spectroscopy showed that most bone tissue adhesion adhered on its surface, more than other two groups. The bionic calcium phosphate coated titanium rods followed, titanium rods at least.Conclusions Simvastatin-loaded biological Ca/P coated Titanium rodContaining simvastatin bionic calcium phosphate coated titanium rods slowly release simvastatin, and significantly can promote new bone ingrowth, bone connecting surface adhesion. This Simvastatin-loaded biological Ca/P coated contains simvastatin biological biomimetic calcium phosphate coating, in artificial joints might have a great potential in joint replacementbroad range of application prospects.